Cloned (Comment) | Organism |
---|---|
recombinant overexpression of His6-SUMO-tagged linked and unlinked NS3-NS2B(45-95) complex linked via G4SG4 linker in Escherichia coli strain BL21 Codon Plus (DE3) | Zika virus |
Protein Variants | Comment | Organism |
---|---|---|
R95*A/R29G | construction of a complex containing residues 45 to 95 of the NS2B cofactor linked via a Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly (G4SG4) linker to the NS3 protease to avoid auto-proteolysis and create the linked protease, the last arginine residue of the NS2B cofactor before the G4SG4 linker is mutated to alanine (R95*A), and arginine 29 of the NS3 protease domain is mutated to glycine (R29G) | Zika virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N2-(thiophen-2-ylcarbonyl)-L-arginyl-N-[(1S)-2-amino-2-oxo-1-(4-[[4-(trifluoromethyl)benzyl]oxy]phenyl)ethyl]-L-lysinamide | the compound inhibits both unlinked and linked protease with similar potency | Zika virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zika virus | A0A384KRS4 | genome polyprotein; ZIKV, the ZIKV isolate is from Puerto Rico, which stems from the Asian lineage and is relevant to the 2015-2016 outbreak in North and South America | - |
Zika virus Puerto Rico isolate | A0A384KRS4 | genome polyprotein; ZIKV, the ZIKV isolate is from Puerto Rico, which stems from the Asian lineage and is relevant to the 2015-2016 outbreak in North and South America | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-SUMO-tagged linked and unlinked NS3-NS2B(45-95) complex linked from Escherichia coli strain BL21 Codon Plus (DE3) by nickel affinity chromatography, tag cleavage by yeast SUMO Protease 1, followed by another step of nickel affinity chromatography, and gel filtration | Zika virus |
Synonyms | Comment | Organism |
---|---|---|
NS2B/NS3 protease | - |
Zika virus |
ZIKV NS2B/NS3 protease | - |
Zika virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Zika virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Zika virus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NS2B cofactor | the NS2B cofactor is covalently linked to the NS3 protease domain via a Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly (G4SG4) linker | Zika virus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00104 | - |
recombinant linked protease, pH 8.5, 37°C | Zika virus | N2-(thiophen-2-ylcarbonyl)-L-arginyl-N-[(1S)-2-amino-2-oxo-1-(4-[[4-(trifluoromethyl)benzyl]oxy]phenyl)ethyl]-L-lysinamide | |
0.00106 | - |
recombinant unlinked protease, pH 8.5, 37°C | Zika virus | N2-(thiophen-2-ylcarbonyl)-L-arginyl-N-[(1S)-2-amino-2-oxo-1-(4-[[4-(trifluoromethyl)benzyl]oxy]phenyl)ethyl]-L-lysinamide |
General Information | Comment | Organism |
---|---|---|
additional information | increased activity of unlinked Zika virus NS2B/NS3 protease compared to linked Zika virus protease. Enzyme structure homology modeling and molecular dynamics simulations of linked and unlinked protease, overview | Zika virus |
physiological function | the ZIKV NS2B/NS3 protease is responsible for cleavage of the viral polyprotein at five sites | Zika virus |