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Literature summary for 3.4.21.91 extracted from

  • Salaemae, W.; Junaid, M.; Angsuthanasombat, C.; Katzenmeier, G.
    Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3 (2010), J. Biomed. Sci., 17, 68.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.91

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Dengue virus

Protein Variants

Protein Variants Comment Organism
D129A mutant shows 39fold reduction in catalytic efficacy compared to wild-type Dengue virus
G133A mutant shows 10fold reduced catalytic activity Dengue virus
G151A inactive enzyme Dengue virus
I165A mutant shows a drastic decrease in catalytic efficacy and a drastic increase in Km Dengue virus
L115A mutant shows activity comparable to wild-type Dengue virus
N152A mutant shows no a complete inactivation but a 60fold reduced catalytic activity Dengue virus
S163A mutant shows 12fold reduction in catalytic efficacy compared to wild-type Dengue virus
T134A mutant shows 2.2fold reduction in catalytic efficacy compared to wild-type Dengue virus
Y150A inactive enzyme Dengue virus

Organism

Organism UniProt Comment Textmining
Dengue virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal affinity chromatography and gel filtration Dengue virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Gly-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
NS2B/NS3 proteinase
-
 Dengue virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Dengue virus

pH Range

pH Minimum pH Maximum Comment Organism
9
-
 assay at Dengue virus