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Literature summary for 3.4.21.91 extracted from

  • Kondo, M.Y.; Oliveira, L.C.; Okamoto, D.N.; de Araujo, M.R.; Duarte dos Santos, C.N.; Juliano, M.A.; Juliano, L.; Gouvea, I.E.
    Yellow fever virus NS2B/NS3 protease: hydrolytic properties and substrate specificity (2011), Biochem. Biophys. Res. Commun., 407, 640-644.
    View publication on PubMed
Search for more literature for 3.4.21.91

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0006
-
 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0015
-
 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.002
-
 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0021
-
 o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0022
-
 SSRKRRSHDVLTQ pH 9.0, 37°C Yellow fever virus
0.0025
-
 MKTGRRGSANGKQ pH 9.0, 37°C Yellow fever virus
0.0027
-
 KLAQRRVFHGVAQ pH 9.0, 37°C Yellow fever virus
0.005
-
 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0066
-
 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0073
-
 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0076
-
 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0085
-
 VRGARRSGDVLWQ pH 9.0, 37°C Yellow fever virus
0.0087
-
 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.0088
-
 SAAQRRGRIGRNQ pH 9.0, 37°C Yellow fever virus
0.0096
-
 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.012
-
 o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.014
-
 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.015
-
 FAEGRRGAAEVLQ pH 9.0, 37°C Yellow fever virus
0.02
-
 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.02
-
 RIFGRRSIPVNEQ pH 9.0, 37°C Yellow fever virus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl kcat/Km values decrease by adding NaCl up to 100 mM. This results from the systematic increase in Km values with the increase in NaCl concentration in the range 1 to 10 mM Yellow fever virus

Organism

Organism UniProt Comment Textmining
Yellow fever virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAEGRRGAAEVLQ + H2O
-
 Yellow fever virus FAEGRR + GAAEVLQ
-
 ?
KLAQRRVFHGVAQ + H2O
-
 Yellow fever virus KLAQRR + VFHGVAQ
-
 ?
MKTGRRGSANGKQ + H2O
-
 Yellow fever virus MKTGRR + GSANGKQ
-
 ?
o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AGKK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AGKR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AGRK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AGRR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AKKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AKKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AKRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-AKRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-ARKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-ARKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-ARRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-ARRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-KKR + SSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Yellow fever virus o-aminobenzoyl-KRR + SSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
RIFGRRSIPVNEQ + H2O
-
 Yellow fever virus RIFGRR + SIPVNEQ
-
 ?
SAAQRRGRIGRNQ + H2O
-
 Yellow fever virus SAAQRRGRIGRNQ
-
 ?
SSRKRRSHDVLTQ + H2O
-
 Yellow fever virus SSRKRR + SHDVLTQ
-
 ?
VRGARRSGDVLWQ + H2O
-
 Yellow fever virus VRGARR + SGDVLWQ
-
 ?

Synonyms

Synonyms Comment Organism
YFV NS2B/NS3 protease
-
 Yellow fever virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Yellow fever virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.001
-
 FAEGRRGAAEVLQ pH 9.0, 37°C Yellow fever virus
0.002
-
 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.003
-
 KLAQRRVFHGVAQ pH 9.0, 37°C Yellow fever virus
0.004
-
 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.005
-
 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.005
-
 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.005
-
 RIFGRRSIPVNEQ pH 9.0, 37°C Yellow fever virus
0.01
-
 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.012
-
 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.012
-
 MKTGRRGSANGKQ pH 9.0, 37°C Yellow fever virus
0.012
-
 o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.016
-
 SAAQRRGRIGRNQ pH 9.0, 37°C Yellow fever virus
0.025
-
 o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.026
-
 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.041
-
 VRGARRSGDVLWQ pH 9.0, 37°C Yellow fever virus
0.06
-
 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.061
-
 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.069
-
 SSRKRRSHDVLTQ pH 9.0, 37°C Yellow fever virus
0.097
-
 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.12
-
 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
 Yellow fever virus

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 enzyme retains more than 90% of its initial protease activity Yellow fever virus
7
-
 Km value increases 5times when pH range is lowered from 8.5 to 7 Yellow fever virus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
 FAEGRRGAAEVLQ pH 9.0, 37°C Yellow fever virus
0.11
-
 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
0.25
-
 RIFGRRSIPVNEQ pH 9.0, 37°C Yellow fever virus
0.6
-
 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
1
-
 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
1
-
 o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
1.1
-
 KLAQRRVFHGVAQ pH 9.0, 37°C Yellow fever virus
1.37
-
 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
1.8
-
 SAAQRRGRIGRNQ pH 9.0, 37°C Yellow fever virus
3.04
-
 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
3.33
-
 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
4.08
-
 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
4.8
-
 VRGARRSGDVLWQ pH 9.0, 37°C Yellow fever virus
4.8
-
 MKTGRRGSANGKQ pH 9.0, 37°C Yellow fever virus
11.9
-
 o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
12.5
-
 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
12.8
-
 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
13
-
 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
18.16
-
 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
20
-
 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine pH 9.0, 37°C Yellow fever virus
31.4
-
 SSRKRRSHDVLTQ pH 9.0, 37°C Yellow fever virus