KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.146 | - |
Gly-Arg-Arg-4-methylcoumarin 7-amide | pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor | Dengue virus type 2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus type 2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Dengue virus type 2 | Gly-Arg-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS3 protease | - |
Dengue virus type 2 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Gly-Arg-Arg-4-methylcoumarin 7-amide | pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor | Dengue virus type 2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | characterization of structural determinants necessary for activation of the enzyme by the NS2B cofactor. Alanine substitutions at residues Trp62, Leu75, and Ile79 in the NS2B cofactor result in marked effects on autoprocessing at the NS2B/NS3 site. The activity of the mutant NS3 protease with the synthetic peptide substrate Gly-Arg-Arg-4-methylcoumarin 7-amide is mainly affected by significantly reduced kcat values | Dengue virus type 2 |