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Literature summary for 3.4.21.90 extracted from
- Role of sindbis virus capsid protein region II in nucleocapsid core assembly and encapsidation of genomic RNA (2008), J. Virol., 82, 4461-4470.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| peptide comprising amino acid residues 81 to 112 of the capsid protein expressed as an Smt3 fusion protein from a modified pET28b vector | Sindbis virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| CDELTA(101-105) | core accumulation, no encapsidation specificity | Sindbis virus |
| CDELTA(101-110) | no core accumulation, no encapsidation specificity | Sindbis virus |
| CDELTA(106-110) | no core accumulation, no encapsidation specificity | Sindbis virus |
| CDELTA(107-113) | no core accumulation, no encapsidation specificity | Sindbis virus |
| CDELTA(91-100) | no core accumulation, no encapsidation specificity | Sindbis virus |
| CDELTA(96-100) | core accumulation, no encapsidation specificity | Sindbis virus |
| CDELTA(97-106) | core accumulation, no encapsidation specificity | Sindbis virus |
| K102A | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K109A | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K83A | binding affinity for encapsidation signal RNA comparable to the wild-type | Sindbis virus |
| K86A | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K92A | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K97A | binding affinity for encapsidation signal RNA comparable to the wild-type | Sindbis virus |
| K97A/K99A | 10fold reduced binding affinity compared to that of the wild type | Sindbis virus |
| K99A | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K99E | accumulates wild-type levels of nucleocapsid cores while still encapsidating approximately 65% viral RNA | Sindbis virus |
| K99E/R105A | accumulates wild-type levels of nucleocapsid cores while still encapsidating approximately 65% viral RNA | Sindbis virus |
| K99L | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K99M | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| K99R | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| L108A | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| L108D | no core accumulation, no encapsidation specificity | Sindbis virus |
| L110A | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| L110N | no core accumulation, no encapsidation specificity | Sindbis virus |
| P100A | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| Q104A | binding affinity for encapsidation signal RNA comparable to the wild-type | Sindbis virus |
| Q88A | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| Q94A | binding affinity for encapsidation signal RNA comparable to the wild-type | Sindbis virus |
| R103A | core accumulation, no encapsidation specificity | Sindbis virus |
| R103A/R105A | 100fold reduced binding affinity compared to that of the wild type | Sindbis virus |
| R105A | core accumulation, no encapsidation specificity | Sindbis virus |
| R105E | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| R105K | stronger binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| R105L | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
| R105M | weaker binding affinity for encapsidation signal RNA than the wild-type | Sindbis virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sindbis virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| capsid protein(81-112) mutant, on Ni-agarose affinity column | Sindbis virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the region that is responsible for nucleocapsid core accumulation has considerable overlap with the region that controls encapsidation specificity. Amino acids 96 to 113 are responsible for the specific encapsidation of genomic RNA. Amino acids 99 and 105 may interact directly with the encapsidation signal. Amino acids 106 to 113 are responsible for nucleocapsid core accumulation | Sindbis virus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| capsid protein | - |
Sindbis virus |
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