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Literature summary for 3.4.21.9 extracted from

  • Melicherova, K.; Krahulec, J.; Safranek, M.; Liskova, V.; Hopkova, D.; Szeliova, D.; Turna, J.
    Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris (2017), Appl. Microbiol. Biotechnol., 101, 1927-1934 .
    View publication on PubMed
Search for more literature for 3.4.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain Y11430 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.108
-
 Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide at pH 8.0 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P98073
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide + H2O
-
 Homo sapiens Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
EKL
-
 Homo sapiens
enterokinase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
58.38
-
 Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide at pH 8.0 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
540.56
-
 Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide at pH 8.0 and 37°C Homo sapiens