Literature summary for 3.4.21.9 extracted from

Pepeliaev, S.; Krahulec, J.; ?erny, Z.; Jilkova, J.; Tlusta, M.; Dostalova, J.
High level expression of human enteropeptidase light chain in Pichia pastoris (2011), J. Biotechnol., 156, 67-75.

Search for more literature for 3.4.21.9

Application

Application	Comment	Organism
molecular biology	the high specificity of the target site makes enterokinase an ideal tool for cleaving fusion proteins at defined cleavage sites	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
the synthetic gene encoding human enteropeptidase light chain with His-tag added at the C-terminus to facilitate protein purification is cloned into Pichia pastoris expression plasmids under the control of an inducible AOX1 or constitutive promoters GAP and AAC	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	x * 27000, deglycosylated recombinant enzyme, SDS-PAGE, x * 37000-72000, hyperglycosylated recombinant enzyme, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trypsinogen + H2O	Homo sapiens	activation by by endoproteolytic cleavage	trypsin	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P98073	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
small intestine	enterokinase is expressed in the proximal part, the heavy chain anchors the enzyme in the intestinal brush border membrane	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trypsinogen + H2O	activation by by endoproteolytic cleavage	Homo sapiens	trypsin	-	?
trypsinogen + H2O	the enzyme cleaves after lysine residue if the Lys is preceded by four Asp and not followed by a Pro, and it shows high specificity of the target site. The light chain represents the catalytic enzyme subunit	Homo sapiens	trypsin	-	?

Subunits

Subunits	Comment	Organism
?	x * 27000, deglycosylated recombinant enzyme, SDS-PAGE, x * 37000-72000, hyperglycosylated recombinant enzyme, SDS-PAGE	Homo sapiens
More	the mature active enzyme is comprised of two disulfide-linked polypeptide chains. The heavy chain anchors the enzyme in the intestinal brush border membrane, whereas the light chain represents the catalytic enzyme subunit	Homo sapiens

Synonyms

Synonyms	Comment	Organism
enterokinase	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	the enzyme converts the inactive form of trypsinogen to active trypsin by endoproteolytic cleavage	Homo sapiens

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Release 2023.1 (January 2023)