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Literature summary for 3.4.21.9 extracted from
- Expression of recombinant chinese bovine enterokinase catalytic subunit in P. pastoris and its purification and characterization (2004), Acta Biochim. Biophys. Sin., 36, 513-517.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | tool protease in the research and production of gene engineering | Bos taurus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris methylotrophic strain GS115 | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE, catalytic subunit of Chinese bovine enterokinase | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+ affinity chromatography | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GST-vasostatin fusion protein + H2O | - |
Bos taurus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Chinese bovine enterokinase | catalytic subunit | Bos taurus |
| EKL-His6 | - |
Bos taurus |
| enterokinase | - |
Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 45 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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