Literature summary for 3.4.21.89 extracted from

Luo, C.; Roussel, P.; Dreier, J.; Page, M.G.; Paetzel, M.
Crystallographic analysis of bacterial signal peptidase in ternary complex with arylomycin A2 and a beta-sultam inhibitor (2009), Biochemistry, 48, 8976-8984.

Search for more literature for 3.4.21.89

Application

Application	Comment	Organism
drug development	SPase I is a target for development of inhibitors as antibiotics	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
soluble catalytic domain of SSPase I in ternary complex with inhibitors arylomycin A2 and BAL0019193, sitting drop vapor diffusion method at 18°C, mixing of 0.002 ml of protein solution and of reservoir solution, containing 0.2 M ammonium formate, 25% PEG 2000, 0.1 M sodium cacodylate pH 6.5, and 5% tertiary-amyl alcohol, equilibration over 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement	Escherichia coli

Crystallization (Comment)

Organism

soluble catalytic domain of SSPase I in ternary complex with inhibitors arylomycin A2 and BAL0019193, sitting drop vapor diffusion method at 18°C, mixing of 0.002 ml of protein solution and of reservoir solution, containing 0.2 M ammonium formate, 25% PEG 2000, 0.1 M sodium cacodylate pH 6.5, and 5% tertiary-amyl alcohol, equilibration over 1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement

Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
arylomycin A2	a lipohexapeptide-based natural product, inhibits SPase I by binding to non-overlapping subsites near the catalytic center in a noncovalent manner, binding mode, overview	Escherichia coli
BAL0019193	a morpholino-beta-sultam derivative, inhibits SPase I by binding to non-overlapping subsites near the catalytic center in a noncovalent manner, binding mode, overview	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	-	Escherichia coli	5783	-

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00803	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	SPase I is an essential membrane-bound endopeptidase with a unique Ser/Lys dyad mechanism	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
Spase I	-	Escherichia coli
type I signal peptidase	-	Escherichia coli