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Literature summary for 3.4.21.89 extracted from
- Signal peptidase I-mediated processing of an engineered mammalian cytochrome b(5) precursor is an exocytoplasmic post-translocational event in Escherichia coli (2008), Mol. Membr. Biol., 25, 388-399.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid pMC-Spase encoding signal peptidase transformed and amplified in Escherichia coli TB1 | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | transcytoplasmic signal peptidase anchored to the inner membrane | Escherichia coli | 5737 | - |
| membrane | transcytoplasmic signal peptidase anchored to the inner membrane | Escherichia coli | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P00803 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by sonication, centrifugation and gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mammalian cytochrome b(5) precursor + H2O | the processing can occur after almost complete exocytoplasmic translocation of the preprotein is accomplished | Escherichia coli | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| signal peptidase I | - |
Escherichia coli |
| SPase | - |
Escherichia coli |
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