Literature summary for 3.4.21.89 extracted from

Karla, A.; Lively, M.O.; Paetzel, M.; Dalbey, R.
The identification of residues that control signal peptidase cleavage fidelity and substrate specificity (2005), J. Biol. Chem., 280, 6731-6741.

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Protein Variants

Protein Variants	Comment	Organism
D142E	10% of wild-type activity	Escherichia coli
D142I	as active as wild-type enzyme	Escherichia coli
F84A	50% of wild-type activity	Escherichia coli
F84W	10% of wild-type activity	Escherichia coli
G89A	as active as wild-type enzyme	Escherichia coli
I130A	as active as wild-type enzyme	Escherichia coli
I144A	50% of wild-type activity	Escherichia coli
I144A/I86A	0.1% of wild-type activity	Escherichia coli
I144C	50% of wild-type activity, mutant enzyme cleaves at multiple sites	Escherichia coli
I144S	10% of wild-type activity	Escherichia coli
I86A	0.5% of wild-type activity	Escherichia coli
I86A/I144A	mutant enzyme is able to cleave after Phe at the -1 residue	Escherichia coli
L95A	10% of wild-type activity	Escherichia coli
L95R	no activity	Escherichia coli
M91A	5% of wild-type activity	Escherichia coli
V132A	1% of wild-type activity	Escherichia coli
V132I	as active as wild-type enzyme	Escherichia coli
Y143A	10% of wild-type activity	Escherichia coli
Y143W	as active as wild-type enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00803	-	-

Synonyms

Synonyms	Comment	Organism
Signal peptidase	-	Escherichia coli
SPase	-	Escherichia coli

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Release 2023.1 (January 2023)