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Literature summary for 3.4.21.89 extracted from

  • Kim, Y.T.; Kurita, R.; Kojima, M.; Nishii, W.; Tanokura, M.; Muramatsu, T.; Ito, H.; Takahashi, K.
    Identification of arginine residues important for the activity of Escherichia coli signal peptidase I (2004), Biol. Chem., 385, 381-388.
    View publication on PubMed
Search for more literature for 3.4.21.89

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzymes Escherichia coli

Protein Variants

Protein Variants Comment Organism
R222A kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme Escherichia coli
R222K kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme Escherichia coli
R315A kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme Escherichia coli
R318A kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value Escherichia coli
R318K kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme Escherichia coli
R77A kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value Escherichia coli
R77K kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R318A Escherichia coli
0.49
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R127A Escherichia coli
0.49
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R275A Escherichia coli
0.49
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R282A Escherichia coli
0.49
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R315K Escherichia coli
0.52
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R146A Escherichia coli
0.52
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R198A Escherichia coli
0.52
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R226A Escherichia coli
0.52
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R236A Escherichia coli
0.52
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R295A Escherichia coli
0.52
-
 FSASALAKI 37°C, pH 7.7, wild-type enzyme Escherichia coli
0.53
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R222K Escherichia coli
0.53
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R318A Escherichia coli
0.54
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R222A Escherichia coli
0.55
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R199A Escherichia coli
0.57
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R315A Escherichia coli
0.63
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R77K Escherichia coli
0.67
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R77A Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, wild-type and mutant enzymes, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FSASALAKI + H2O
-
 Escherichia coli FSASALA + Lys-Ile
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, wild-type and mutant enzymes, SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R318A Escherichia coli
0.08
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R318A Escherichia coli
0.248
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R77A Escherichia coli
0.27
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R222A Escherichia coli
0.32
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R222K Escherichia coli
0.42
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R315A Escherichia coli
0.71
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R77K Escherichia coli
0.96
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R226A Escherichia coli
1
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R275A Escherichia coli
1.02
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R315K Escherichia coli
1.11
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R127A Escherichia coli
1.11
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R198A Escherichia coli
1.2
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R282A Escherichia coli
1.2
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R295A Escherichia coli
1.31
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R199A Escherichia coli
1.31
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R236A Escherichia coli
1.44
-
 FSASALAKI 37°C, pH 7.7, mutant enzyme R146A Escherichia coli
1.44
-
 FSASALAKI 37°C, pH 7.7, wild-type enzyme Escherichia coli