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Literature summary for 3.4.21.89 extracted from

  • Bruton, G.; Huxley, A.; O'Hanlon, P.; Orlek, B.; Eggleston, D.; Humphries, J.; Readshaw, S.; West, A.; Ashman, S.; Brown, M.; Moore, K.; Pope, A.; O'Dwyer, K.; Wang, L.
    Lipopeptide substrates for SpsB, the Staphylococcus aureus type I signal peptidase: design, conformation and conversion to alpha-ketoamide inhibitors (2003), Eur. J. Med. Chem., 38, 351-356.
    View publication on PubMed
Search for more literature for 3.4.21.89

Application

Application Comment Organism
pharmacology the gram positive pathogen plays a significant role in infectious disease, including life threatening methicillin-resistant MRSA infections, the ability to screen for inhibitors of SpsB will represent a significant advance for discovery of antibacterial agents Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
decanoyl-LTPTA susceptible to classic protease inhibition, SpsB can be inhibited with a P1' proline Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 decanoyl-LTPTAKAASKIDD-OH pH 8.5, 37°C Staphylococcus aureus
0.988
-
 decanoyl-LT-P-TAKA-A-SKIDD-OH pH 8.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
signal peptides from preproteins + H2O Staphylococcus aureus
-
 mature proteins
-
 ?
signal peptides from preproteins + H2O Escherichia coli
-
 mature proteins
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
decanoyl-LTPTAKAASKIDD-OH + H2O
-
 Staphylococcus aureus decanoyl-LTPTAKA + ASKIDD
-
 ?
decanoyl-LTPTAKAASKIDD-OH + H2O
-
 Escherichia coli decanoyl-LTPTAKA + ASKIDD
-
 ?
hexanoyl-LTPTQAKAASKIDD-OH + H2O
-
 Staphylococcus aureus hexanoyl-LTPTQAKA + ASKIDD
-
 ?
octanoyl-LTPTQAKAASKIDD-OH + H2O
-
 Staphylococcus aureus octanoyl-LTPTQAKA + ASKIDD
-
 ?
pro-ompA-nuclease + H2O
-
 Staphylococcus aureus ompA-nuclease + ?
-
 ?
pro-ompA-nuclease + H2O
-
 Escherichia coli ompA-nuclease + ?
-
 ?
signal peptides from preproteins + H2O
-
 Staphylococcus aureus mature proteins
-
 ?
signal peptides from preproteins + H2O
-
 Escherichia coli mature proteins
-
 ?

Synonyms

Synonyms Comment Organism
SpsB
-
 Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
67
-
 decanoyl-LTPTAKAASKIDD-OH pH 8.5, 37°C Staphylococcus aureus
418
-
 decanoyl-LTPTAKAASKIDD-OH pH 8.5, 37°C Staphylococcus aureus