Literature summary for 3.4.21.89 extracted from

Zheng, F.; Angleton, E.L.; Lu, J.; Peng, S.B.
In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I (2002), Eur. J. Biochem., 269, 3969-3977.

Search for more literature for 3.4.21.89

Cloned(Commentary)

Cloned (Comment)	Organism
expression vector pET16b-spi directs synthesis of full length SPase I, transformed into Escherichia coli BL21(DE3)pLysS and overexpressed	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
signal peptides from preproteins + H2O	Streptococcus pneumoniae	-	mature proteins	-	?
streptokinase precursor + H2O	Streptococcus pneumoniae	prestreptokinase is the native substrate, to be cleaved between Ala26 and Ile27	streptokinase	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Streptococcus pneumoniae

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
KLTFGTVKPVQAIAGYEWL + H2O	synthetic peptide substrate, based upon the signal peptide of prestreptokinase from Streptococcus pyogene	Streptococcus pneumoniae	?	-	?
additional information	precursor of beta-lactamase and pre-OmpA fusion protein are no substrates, incubation at 37°C results in self-cleavage and appearance of 2 products with molecular masses of 19 kDa and 8 kDa	Streptococcus pneumoniae	?	-	?
signal peptidase I + H2O	self-cleavage, results in a truncated product	Streptococcus pneumoniae	SPase37-204	-	?
signal peptides from preproteins + H2O	-	Streptococcus pneumoniae	mature proteins	-	?
streptokinase precursor + H2O	extracellular protein, produced in pathogenic streptococci prestreptokinase is the native substrate	Streptococcus pneumoniae	streptokinase	-	?
streptokinase precursor + H2O	prestreptokinase is the native substrate, to be cleaved between Ala26 and Ile27	Streptococcus pneumoniae	streptokinase	-	?

Synonyms

Synonyms	Comment	Organism
SPaseI	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)