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Literature summary for 3.4.21.89 extracted from
- Leader peptidase from Escherichia coli: overexpression, characterization, and inactivation by modification of tryptophan residues 300 and 310 with N-bromosuccinimide (1995), J. Biochem., 117, 535-544.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | - |
Escherichia coli |  |
| Bromosuccinimide | inactivation by modification of tryptophan residues 300 and 310 | Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| diethyldicarbonate | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| additional information | scarcely inhibited by treatment with: N-acetylimidazole, iodoacetic acid, 5,5'-dithiobis(2-nitrobenzoic acid), succinic anhydride, 2,4,6-trinitrobenzenesulfonate | Escherichia coli | |
| Phenylglyoxal | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile + H2O | - |
Escherichia coli | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
1 h, pH 7, stable below | Escherichia coli |
| 50 | - |
1 h, pH 7, complete inactivation | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | 10 | 7.7: 57% of activity maximum, 10: maximum activity | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10.5 | 37°C, 30 min, more than 70% of the activity is retained | Escherichia coli |
| 11 | - |
inactivated nearly completely at above pH 11.0 | Escherichia coli |
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