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Literature summary for 3.4.21.89 extracted from

  • Wolfe, P.B.; Zwizinski, C.; Wickner, W.
    Purification and characterization of leader peptidase from Escherichia coli (1983), Methods Enzymol., 97, 40-46.
    View publication on PubMed
Search for more literature for 3.4.21.89

General Stability

General Stability Organism
Stable at low ionic strength in solutions of nonionic detergents at low temperature Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Carboxyphenanthroline
-
 Escherichia coli
cholate
-
 Escherichia coli
deoxycholate
-
 Escherichia coli
dinitrophenol
-
 Escherichia coli
Mg2+ above 1 mM Escherichia coli
NEM
-
 Escherichia coli
SDS
-
 Escherichia coli
sodium chloride above 160 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 1 * 37000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
even after boiling in SDS, by dilution in Triton X-100 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
M13 phage procoat protein + H2O hydrolysis of a single-Ala-+-Ala- bond, the term-+- depicts the point of cleavage Escherichia coli Free signal peptide + coat protein
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.9 Escherichia coli
8 9
-
 Escherichia coli