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Literature summary for 3.4.21.89 extracted from

  • Tschantz, W.R.; Dalbey, R.E.
    Bacterial leader peptidase 1 (1994), Methods Enzymol., 244, 285-301.
    View publication on PubMed
Search for more literature for 3.4.21.89

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ above 1 mM Escherichia coli
additional information but site-directed mutagenesis implicates a Ser/Lys catalytic dyad in activity; unaffected by inhibitors of most serine peptidases Escherichia coli
sodium chloride above 160 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile
-
 Escherichia coli
1
-
 Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2
-
 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Escherichia coli 16020
-
membrane it is anchored to the membrane by two transmembrane segments and is oriented with a short domain facing the cytoplasm and a large carboxyl-terminal domain exposed to the periplasmic space Escherichia coli 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 1 * 36000 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the physiological role is to release exported proteins from the membrane by removing the leader sequence ?
-
 ?
additional information Escherichia coli MC1061/pRD8 the physiological role is to release exported proteins from the membrane by removing the leader sequence ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MC1061/pRD8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
M13 phage procoat protein + H2O hydrolysis of a single-Ala-+-Ala- bond, the term-+- depicts the point of cleavage Escherichia coli Free signal peptide + coat protein
-
 ?
M13 phage procoat protein + H2O hydrolysis of a single-Ala-+-Ala- bond, the term-+- depicts the point of cleavage Escherichia coli MC1061/pRD8 Free signal peptide + coat protein
-
 ?
additional information specificity: leader peptidase 1 cleaves the majority of the preproteins destined to the cell surface Escherichia coli ?
-
 ?
additional information the physiological role is to release exported proteins from the membrane by removing the leader sequence Escherichia coli ?
-
 ?
additional information specificity: leader peptidase 1 cleaves the majority of the preproteins destined to the cell surface Escherichia coli MC1061/pRD8 ?
-
 ?
additional information the physiological role is to release exported proteins from the membrane by removing the leader sequence Escherichia coli MC1061/pRD8 ?
-
 ?
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile + H2O
-
 Escherichia coli Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile + ?
-
 ?
Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2 + H2O
-
 Escherichia coli Phe-Ser-Ala-Ser-Ala-Leu-Ala + Lys-Ile-NH2
-
 ?
Pre-beta-lactamase + H2O
-
 Escherichia coli beta-Lactamase + ?
-
 ?
Pre-beta-lactamase + H2O
-
 Escherichia coli MC1061/pRD8 beta-Lactamase + ?
-
 ?
Pre-lambda phage receptor + H2O
-
 Escherichia coli Lambda Phage receptor + ?
-
 ?
Precursor of the leucine-binding protein + H2O
-
 Escherichia coli Leucine-binding protein + ?
-
 ?
Precursor of the leucine-binding protein + H2O
-
 Escherichia coli MC1061/pRD8 Leucine-binding protein + ?
-
 ?
Premaltose-binding protein + H2O
-
 Escherichia coli Maltose-binding protein + ?
-
 ?
Pro-OmpA + H2O
-
 Escherichia coli OmpA + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0212
-
 Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile
-
 Escherichia coli
0.033
-
 Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.9 Escherichia coli