Literature summary for 3.4.21.88 extracted from

Giese, K.C.; Michalowski, C.B.; Little, J.W.
RecA-dependent cleavage of LexA dimers (2008), J. Mol. Biol., 377, 148-161.

Search for more literature for 3.4.21.88

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli as wild type, mutants and as truncated proteins	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K156A	site directed mutagenesis	Escherichia coli
additional information	K156A, L89P, Q92W, E152A quadruple mutant, K156A, Q92W, E152A mutant variant -89, K156A, L89P, E152A mutant variant -92, K156A, L89P, Q92W mutant variant -152, K156A, Q92W mutant variant, Q92W S119A mutant variant, expression of truncated variants	Escherichia coli
S119A	site directed mutagenesis	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
RecA	DNA repair -inducing treatments activate RecA, which inactivates LexA by a specific cleavage reaction, leading to derepression of the SOS regulon, RecA stimulates the transition of LexA from its noncleavable to its cleavable conformation	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
no activity in Streptococcus thermophilus	-	a small DNA region upstream of the recA transcriptional start site carries all the information needed for normal regulation of the S. thermophilus recA gene	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	crosslinked with glutaraldehyde	Escherichia coli

Purification (Commentary)

Purification (Comment)	Organism
of the recombinant protein und its truncated variants by Ninitrilotriacetic acid column chromatography	Escherichia coli

Subunits

Subunits	Comment	Organism
dimer	under physiological conditions, is cleaved by RecA, dimmers dissociate slowly	Escherichia coli

Synonyms

Synonyms	Comment	Organism
LexA	-	Escherichia coli
LexA	-	no activity in Streptococcus thermophilus
LexA repressor	-	Escherichia coli

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Release 2023.1 (January 2023)