Crystallization (Comment) | Organism |
---|---|
mutants G85D, S119A, L89P/Q92W/E152A/K156A, and tryptic fragments of mutants S119A and L89P/Q92W/E152A/K156A | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G85D | change in cleavage site, that blocks autocleavage but has normal active site, crystallization data | Escherichia coli |
L89P/Q92W/E152A/K156A | designed to trap protein in conformation required for cleavage. Crystallization data of tryptic fragment containing amino acids 68202 | Escherichia coli |
S119A | change in the active site, but normal cleavage site. Crystallization data of full length protein and of tryptic fragment containing amino acids 68202 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | protein undergoes self-cleavage reaction that requires RecA protein or occurs spontaneously at high pH-value. Cleavage site has two distinct and highly ordered conformations | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Hydrolysis of Ala84-/-Gly bond in repressor LexA | oxyanion hole is formed by A84, S119, M118 | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization data, mutants G85D, S119A, L89P/Q92W/E152A/K156A | Escherichia coli |