Literature summary for 3.4.21.83 extracted from

McLuskey, K.; Paterson, N.G.; Bland, N.D.; Isaacs, N.W.; Mottram, J.C.
Crystal structure of Leishmania major oligopeptidase B gives insight into the enzymatic properties of a trypanosomatid virulence factor (2010), J. Biol. Chem., 285, 39249-39259.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Leishmania major

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with the inhibitor antipain and as mercury derivative, to 1.65 and 2.0 A resolution, respectively. OPB comprises two domains, a catalytic and a propeller domain, linked together by a hinge region. Residue E621 plays a critical role in the S1 binding pocket and, along with F603, is largely responsible for the enzyme substrate specificity in P1. In the S2 binding pocket, Y499 is important for substrate stability. Residue E623 forms an inter-domain hydrogen bond	Leishmania major

Crystallization (Comment)

Organism

in complex with the inhibitor antipain and as mercury derivative, to 1.65 and 2.0 A resolution, respectively. OPB comprises two domains, a catalytic and a propeller domain, linked together by a hinge region. Residue E621 plays a critical role in the S1 binding pocket and, along with F603, is largely responsible for the enzyme substrate specificity in P1. In the S2 binding pocket, Y499 is important for substrate stability. Residue E623 forms an inter-domain hydrogen bond

Leishmania major

Inhibitors

Inhibitors	Comment	Organism	Structure
antipain	-	Leishmania major

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.00093	-	N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.0012	-	benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.0031	-	N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.0038	-	N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.0044	-	benzoyl-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	Q4QHU7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
benzoyl-L-Arg-7-amido-4-methylcoumarin + H2O	-	Leishmania major	benzoyl-L-Arg + 7-amino-4-methylcoumarin	-	?
benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O	-	Leishmania major	benzyloxycarbonyl-Gly-L-Arg-L-Arg + 7-amino-4-methylcoumarin	-	?
additional information	no substrate: N-benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin	Leishmania major	?	-	?
N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O	-	Leishmania major	N-benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin	-	?
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O	-	Leishmania major	N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin	-	?
N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O	-	Leishmania major	N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.01	-	N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.011	-	N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.013	-	benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.016	-	benzoyl-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
0.02	-	N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
1	-	benzyloxycarbonyl-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
1.1	-	N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
3.6	-	benzoyl-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
3.6	-	N-benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major
5.3	-	N-benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin	21°C, pH not specified in the publication	Leishmania major