Literature summary for 3.4.21.83 extracted from

Khairullin, R.F.; Mikhailova, A.G.; Sebyakina, T.Y.; Lubenets, N.L.; Ziganshin, R.H.; Demidyuk, I.V.; Gromova, T.Y.; Kostrov, S.V.; Rumsh, L.D.
Oligopeptidase B from Serratia proteamaculans. I. Determination of primary structure, isolation, and purification of wild-type and recombinant enzyme variants (2009), Biochemistry (Moscow), 74, 1164-1172.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL-21(DE3) cells	Serratia proteamaculans

Inhibitors

Inhibitors	Comment	Organism	Structure
methanol	incubation in 20% (v/v) methanol (10-20 h, 4°C) results in partial decomposition of the high molecular weight PSP-GroEL complex with precipitation of denatured chaperonin and a small (no more than 10%) loss of PSP activity	Serratia proteamaculans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78000	-	His-tagged enzyme, SDS-PAGE	Serratia proteamaculans

Organism

Organism	UniProt	Comment	Textmining
Serratia proteamaculans	B3VI58	-	-
Serratia proteamaculans 94	B3VI58	-	-

Purification (Commentary)

Purification (Comment)	Organism
QSepharose column chromatography, ultrafiltration, Ni-NTA-agarose column chromatography, and immobilized basic pancreatic trypsin inhibitor-Sepharose column chromatography	Serratia proteamaculans

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O	-	Serratia proteamaculans	Nalpha-benzoyl-DL-arginine + 4-nitroaniline	-	?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O	-	Serratia proteamaculans 94	Nalpha-benzoyl-DL-arginine + 4-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
oligopeptidase B	-	Serratia proteamaculans
OpdB	-	Serratia proteamaculans
PSP	-	Serratia proteamaculans
trypsin-like protease	-	Serratia proteamaculans

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Release 2023.1 (January 2023)