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Literature summary for 3.4.21.83 extracted from
- Oligopeptidase B: a processing peptidase involved in pathogenesis (2008), Biochimie, 90, 336-344.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | - |
Trypanosoma brucei |  |
| dithiothreitol | - |
Trypanosoma brucei | |
| glutathione | - |
Trypanosoma brucei | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure solved at 2.7 A resolution | Trypanosoma brucei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C559S | mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents | Trypanosoma brucei |
| C597S | mutant shows significant decrease in activation by reducing agents. This residue may thus mediate the activity enhancing effect of reducing agents | Trypanosoma brucei |
| additional information | cys256 is identified as the reactive cysteine residue responsible for inactivation by N-ethylmaleimide and iodoacetic acid | Trypanosoma brucei |
| additional information | mutation of Tyr452 confirmed that this residue is involved in the catalytic mechanism by stabilising the intermediate consisting of an oxoanion. Furthermore, it is shown that this oxanion binding site prevents non-productive substrate binding | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,4-dichloroisocoumarin | non-peptide irreversible serine-peptidase inhibitor | Trypanosoma brucei | |
| 4-(2-aminoethyl)benzenesulfonylfluoride | non-peptide irreversible serine-peptidase inhibitor | Trypanosoma brucei | |
| 4-methylumbelliferyl-p-guanidobenzoate | active site titrant | Trypanosoma brucei | |
| antipain | reversible competitive inhibitor | Trypanosoma brucei | |
| diisopropylfluorophosphate | non-peptide irreversible serine-peptidase inhibitor | Trypanosoma brucei | |
| iodoacetic acid | - |
Trypanosoma brucei | |
| leupeptin | reversible competitive inhibitor | Trypanosoma brucei | |
| additional information | no inhibition by N-ethylmaleimide and iodoacetic acid. This is consistent with the absence of a cysteine residue at position 256 which is replaced by His | Escherichia coli | |
| N-ethylmaleimide | - |
Trypanosoma brucei | |
| p-chloromercuribenzoate | non-peptide irreversible serine-peptidase inhibitor | Trypanosoma brucei | |
| Pentamidine | reversible competitive inhibitor | Trypanosoma brucei | |
| peptidyl chloromethyl ketone | irreversible peptidyl inhibitors | Trypanosoma brucei | |
| peptidyl diazomethyl ketone | irreversible peptidyl inhibitors | Trypanosoma brucei | |
| peptidyl phosphonate alpha-aminoalkyl diphenyl ester | irreversible peptidyl inhibitors | Trypanosoma brucei | |
| protamine | reversible competitive inhibitor | Trypanosoma brucei | |
| protamines | protamines, basic 30-32 residue peptides that are rich in Arg residues, are potent inhibitors of OpdB | Escherichia coli | |
| suramin | partial non-competitive inhibitor | Trypanosoma brucei | |
| Tos-LysCH2Cl | non-peptide irreversible serine-peptidase inhibitor | Trypanosoma brucei | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
- |
Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P24555 | - |
- |
| Moraxella lacunata | Q59536 | - |
- |
| Rhodococcus erythropolis | - |
- |
- |
| Salmonella enterica | - |
- |
- |
| Treponema denticola | - |
- |
- |
| Trypanosoma brucei | Q382P7 | - |
- |
| Trypanosoma cruzi | Q4CW30 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the specific activity of OpdB in Escherichia coli increases under conditions where the carbon levels are limiting | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | cleaves oxidised insulin B chain | Escherichia coli | ? | - |
? | |
| additional information | OpdB does not hydrolyse protein substrates with the exception of nominal digestion of the endogenous Escherichia coli enzymes aspartokinase L-homoserine dehydrogenase and aspartokinase III | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| oligopeptidase B | - |
Rhodococcus erythropolis |
| oligopeptidase B | - |
Salmonella enterica |
| oligopeptidase B | - |
Treponema denticola |
| oligopeptidase B | - |
Moraxella lacunata |
| oligopeptidase B | - |
Trypanosoma brucei |
| oligopeptidase B | - |
Trypanosoma cruzi |
| oligopeptidase B | - |
Escherichia coli |
| OP-Tb | - |
Trypanosoma brucei |
| Opd B | - |
Rhodococcus erythropolis |
| Opd B | - |
Salmonella enterica |
| Opd B | - |
Treponema denticola |
| Opd B | - |
Moraxella lacunata |
| Opd B | - |
Trypanosoma cruzi |
| Opd B | - |
Escherichia coli |
| Protease II | - |
Rhodococcus erythropolis |
| Protease II | - |
Salmonella enterica |
| Protease II | - |
Treponema denticola |
| Protease II | - |
Moraxella lacunata |
| Protease II | - |
Escherichia coli |
| Tc 120 | - |
Trypanosoma cruzi |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000181 | - |
antipain | - |
Trypanosoma brucei | |
| 0.00001 | - |
protamine | - |
Trypanosoma brucei | |
| 0.00003 | - |
leupeptin | - |
Trypanosoma brucei | |
| 0.0034 | - |
Pentamidine | - |
Trypanosoma brucei | |
| 0.0067 | - |
suramin | - |
Trypanosoma brucei | |
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Release 2023.1 (January 2023)
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