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Literature summary for 3.4.21.83 extracted from

  • Morty, R.E.; Pelle, R.; Vadasz, I.; Uzcanga, G.L.; Seeger, W.; Bubis, J.
    Oligopeptidase B from Trypanosoma evansi. A parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts (2005), J. Biol. Chem., 280, 10925-10937.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Trypanosoma evansi

Protein Variants

Protein Variants Comment Organism
S563A catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin Trypanosoma evansi

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin irreversible Trypanosoma evansi
4-(2-aminoethyl)benzenesulfonyl fluoride irreversible Trypanosoma evansi
agmatine reversible Trypanosoma evansi
antipain reversible Trypanosoma evansi
benzamidine reversible Trypanosoma evansi
leupeptin reversible Trypanosoma evansi
phenylmethanesulfonyl fluoride irreversible Trypanosoma evansi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0002
-
Val-Lys-Arg-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0003
-
Val-Lys-Arg-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0006
-
Arg-Arg-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0008
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.001
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.001
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0011
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0011
-
Arg-Arg-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0011
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0012
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0012
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0013
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0013
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0016
-
Gly-Gly-Lys-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0017
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0019
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0019
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.002
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0021
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0022
-
Gly-Gly-Lys-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0023
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0024
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0027
-
Phe-Arg-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0029
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.003
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.003
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.003
-
Phe-Arg-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.003
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.003
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0033
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0038
-
Phe-Arg-2-naphthylamide pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.004
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0044
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0044
-
Ala-Phe-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0047
-
Gly-Arg-2-naphthylamide pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0048
-
Gly-Arg-2-naphthylamide pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0049
-
Phe-Arg-2-naphthylamide pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.005
-
Ala-Phe-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0102
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.0122
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0135
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.0143
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.04
-
Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.048
-
Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Atrial natriuretic factor + H2O Trypanosoma evansi cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi ?
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma evansi Q6QDP1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trypanosoma evansi

Source Tissue

Source Tissue Comment Organism Textmining
additional information Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts Trypanosoma evansi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.017
-
-
Trypanosoma evansi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
-
Trypanosoma evansi 3-(2-furyl)acryloyl-Tyr-Lys + Arg
-
?
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
Arg-Arg-2-naphthylamide + H2O
-
Trypanosoma evansi ?
-
?
Arg-Arg-Arg-OH + H2O
-
Trypanosoma evansi Arg-Arg + Arg
-
?
Atrial natriuretic factor + H2O cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi Trypanosoma evansi ?
-
?
Atrial natriuretic factor + H2O cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor Trypanosoma evansi ?
-
?
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
Gly-Arg-2-naphthylamide + H2O
-
Trypanosoma evansi ?
-
?
Gly-Gly-Lys-Arg + H2O
-
Trypanosoma evansi Gly-Gly-Lys-Arg + Arg
-
?
Phe-Arg-2-naphthylamide + H2O
-
Trypanosoma evansi ?
-
?
Phe-Arg-Arg + H2O
-
Trypanosoma evansi Phe-Arg + Arg
-
?
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
Trypanosoma evansi ?
-
?
Val-Lys-Arg-Arg + H2O
-
Trypanosoma evansi Val-Lys-Arg + Arg
-
?

Synonyms

Synonyms Comment Organism
OpdB
-
Trypanosoma evansi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
2
-
Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
8
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
9
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
11
-
Gly-Arg-2-naphthylamide pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
12
-
Gly-Arg-2-naphthylamide pH 8.0, 37°C, native enzyme Trypanosoma evansi
12
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
12
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
12
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
16
-
Phe-Arg-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
17
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
17
-
Phe-Arg-2-naphthylamide pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
18
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
18
-
Phe-Arg-2-naphthylamide pH 8.0, 37°C, native enzyme Trypanosoma evansi
18
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
21
-
Phe-Arg-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
21
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
22
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
23
-
Ala-Phe-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
26
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
29
-
Arg-Arg-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
30
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C, native enzyme Trypanosoma evansi
33
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C,recombinant enzyme Trypanosoma evansi
34
-
Ala-Phe-Lys-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
38
-
Arg-Arg-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
38
-
Val-Lys-Arg-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
40
-
Gly-Gly-Lys-Arg pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
44
-
Val-Lys-Arg-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
48
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
54
-
Gly-Gly-Lys-Arg pH 8.0, 37°C, native enzyme Trypanosoma evansi
55
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C,recombinant enzyme Trypanosoma evansi
57
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
58
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
59
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
59
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
64
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
64
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
74
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
76
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
77
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
81
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
94
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
101
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
102
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
108
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, native enzyme Trypanosoma evansi
122
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, ecombinant mutant enzyme S563A Trypanosoma evansi
132
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
154
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin Trypanosoma evansi

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin Trypanosoma evansi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
antipain pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.002
-
antipain pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.021
-
leupeptin pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.029
-
leupeptin pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.188
-
agmatine pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.207
-
agmatine pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi
0.218
-
benzamidine pH 8.0, 37°C, native enzyme Trypanosoma evansi
0.254
-
benzamidine pH 8.0, 37°C, recombinant enzyme Trypanosoma evansi