Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | 0.3 microM, 103% activity | Xenorhabdus nematophila | |
bestatin | 0.13 mM, 105% activity | Xenorhabdus nematophila | |
E-64 | 5 microM, 114% activity | Xenorhabdus nematophila | |
leupeptin | 1 microM, 116% activity | Xenorhabdus nematophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain dihydrochloride | 1 mM, 79% remaining activity | Xenorhabdus nematophila | |
chymostatin | 0,1 mM, 45% inhibition | Xenorhabdus nematophila | |
EDTA | 1.3 mM, 98% remaining activity, 20 mM, 57% remaining activity | Xenorhabdus nematophila | |
Pefabloc SC | 4 mM, 93% inhibition | Xenorhabdus nematophila | |
pepstatin | 1 microM, 94% remaining activity | Xenorhabdus nematophila | |
phosphoramidon | 0.57 mM, 96% remaining activity | Xenorhabdus nematophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.234 | - |
N-tosyl-Gly-Pro-Arg-paranitroanilide | - |
Xenorhabdus nematophila | |
0.429 | - |
DL-Val-Leu-Arg-paranitroanilide | - |
Xenorhabdus nematophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Xenorhabdus nematophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenorhabdus nematophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenorhabdus nematophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
176 | - |
purified enzyme | Xenorhabdus nematophila |
Storage Stability | Organism |
---|---|
-20°C, stable for several days | Xenorhabdus nematophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Xenorhabdus nematophila | ? | - |
? | |
DL-Val-Leu-Arg-paranitroanilide + H2O | - |
Xenorhabdus nematophila | DL-Val-Leu-Arg + p-nitroaniline | - |
? | |
N-tosyl-Gly-Pro-Arg-paranitroanilide + H2O | - |
Xenorhabdus nematophila | N-tosyl-Gly-Pro-Arg + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Xenorhabdus nematophila |
Synonyms | Comment | Organism |
---|---|---|
Protease II | - |
Xenorhabdus nematophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
- |
Xenorhabdus nematophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
activity is reduced by 90% at 60°C for 10 min | Xenorhabdus nematophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Xenorhabdus nematophila |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Xenorhabdus nematophila | estimated from amino acid sequence | - |
8.5 |