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Literature summary for 3.4.21.83 extracted from

  • Caldas, C.; Cherqui, A.; Pereira, A.; Simoes, N.
    Purification and characterization of an extracellular protease from Xenorhabdus nematophila involved in insect immunosuppression (2002), Appl. Environ. Microbiol., 68, 1297-1304.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Aprotinin 0.3 microM, 103% activity Xenorhabdus nematophila
bestatin 0.13 mM, 105% activity Xenorhabdus nematophila
E-64 5 microM, 114% activity Xenorhabdus nematophila
leupeptin 1 microM, 116% activity Xenorhabdus nematophila

Inhibitors

Inhibitors Comment Organism Structure
antipain dihydrochloride 1 mM, 79% remaining activity Xenorhabdus nematophila
chymostatin 0,1 mM, 45% inhibition Xenorhabdus nematophila
EDTA 1.3 mM, 98% remaining activity, 20 mM, 57% remaining activity Xenorhabdus nematophila
Pefabloc SC 4 mM, 93% inhibition Xenorhabdus nematophila
pepstatin 1 microM, 94% remaining activity Xenorhabdus nematophila
phosphoramidon 0.57 mM, 96% remaining activity Xenorhabdus nematophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.234
-
N-tosyl-Gly-Pro-Arg-paranitroanilide
-
Xenorhabdus nematophila
0.429
-
DL-Val-Leu-Arg-paranitroanilide
-
Xenorhabdus nematophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Xenorhabdus nematophila

Organism

Organism UniProt Comment Textmining
Xenorhabdus nematophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Xenorhabdus nematophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
176
-
purified enzyme Xenorhabdus nematophila

Storage Stability

Storage Stability Organism
-20°C, stable for several days Xenorhabdus nematophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Xenorhabdus nematophila ?
-
?
DL-Val-Leu-Arg-paranitroanilide + H2O
-
Xenorhabdus nematophila DL-Val-Leu-Arg + p-nitroaniline
-
?
N-tosyl-Gly-Pro-Arg-paranitroanilide + H2O
-
Xenorhabdus nematophila N-tosyl-Gly-Pro-Arg + p-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Xenorhabdus nematophila

Synonyms

Synonyms Comment Organism
Protease II
-
Xenorhabdus nematophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
-
Xenorhabdus nematophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
activity is reduced by 90% at 60°C for 10 min Xenorhabdus nematophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Xenorhabdus nematophila

pI Value

Organism Comment pI Value Maximum pI Value
Xenorhabdus nematophila estimated from amino acid sequence
-
8.5