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Literature summary for 3.4.21.83 extracted from
- Role of the oxyanion binding site and subsites S1 and S2 in the catalysis of oligopeptidase B, a novel target for antimicrobial chemotherapy (2002), Biochemistry, 41, 4096-4106.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | involved in host cell invasion, important target for drug design | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli JM83 | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y452F | site-specific mutagenesis | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-Thr-Arg-Arg | - |
Escherichia coli |  |
| benzamidine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C, 3 mM | Escherichia coli | |
| 0.014 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 15°C, 1 M | Escherichia coli | |
| 0.024 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 0.0298 | - |
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 0.0379 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 35°C | Escherichia coli | |
| 0.08 | - |
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C, 1 M | Escherichia coli | |
| 0.083 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation at 5-10 mM CaCl2 | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81860 | - |
amino acid sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | involved in host cell invasion | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| 2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| 2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| 2-aminobenzoyl-Thr-Cit-Cit-Phe(NO2)-Ser-Leu-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-Arg-Et + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-Arg-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-citrulline-Et + H2O | - |
Escherichia coli | ? | - |
? | |
| benzyloxycarbonyl-Arg-Arg-7-(4-methylcoumaryl)amide + H2O | - |
Escherichia coli | ? | - |
? | |
| additional information | specific for substrates with a pair of basic residues at positions P1 and P2 | Escherichia coli | ? | - |
? | |
| additional information | involved in host cell invasion | Escherichia coli | ? | - |
? | |
| Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O | - |
Escherichia coli | Nalpha-benzoyl-L-Arg + 2-naphthylamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
the abnormally low temperature optimum may be caused by the disruption of the hydrogen bond formed between the enzyme and the NH group of the scissile peptide bond | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 294 | - |
2-aminobenzoyl-Thr-Arg Cit-Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 516 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 15°C, 1 M | Escherichia coli | |
| 1218 | - |
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 1220 | - |
2-aminobenzoyl-Thr-Cit-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 1260 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C, 3 mM | Escherichia coli | |
| 1390 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 1392 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 25°C | Escherichia coli | |
| 1416 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 35°C | Escherichia coli | |
| 1420 | - |
2-aminobenzoyl-Thr-Arg-Arg Phe(NO2)-Ser-Leu-NH2 | pH 8.0, 35°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | - |
Escherichia coli |
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