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Literature summary for 3.4.21.83 extracted from
- Oligopeptidase B: a new type of serine peptidase with a unique substrate-dependent temperature sensitivity (1999), Biochemistry, 38, 15548-15555.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzoyl-Arg-4-nitroanilide | enzyme is inhibited by high concentrations of substrate | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.097 | - |
benzoyl-Arg-beta-naphthylamide | pH 8.00, 25°C | Escherichia coli | |
| 0.195 | - |
benzoyl-Arg-4-nitroanilide | pH 7.10, 25°C | Escherichia coli | |
| 0.296 | - |
benzoyl-Arg-4-nitroanilide | pH 8.03, 25°C | Escherichia coli | |
| 0.301 | - |
benzoyl-Arg-4-nitroanilide | pH 8.00, 25°C | Escherichia coli | |
| 1.24 | - |
benzoyl-Arg-4-nitroanilide | pH 8.10, 25°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81860 | - |
amino acid sequence | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Arg-4-nitroanilide + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-Arg-Et + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-Arg-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-arginine ethyl ester + H2O | - |
Escherichia coli | ? | - |
? | |
| benzoyl-citrulline-Et + H2O | - |
Escherichia coli | ? | - |
? | |
| additional information | hydrolyzes peptides with dibasic sites much faster than monobasic substrates, hydrolyzes the peptide bond at lysine and arginine residues | Escherichia coli | ? | - |
? | |
| Nalpha-benzoyl-L-Arg 2-naphthylamide + H2O | - |
Escherichia coli | Nalpha-benzoyl-L-Arg + 2-naphthylamine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
optimum temperature for the reactions of amide substrates slightly below | Escherichia coli |
| 39 | - |
benzoyl-arginine ethyl ester | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4848 | - |
benzoyl-Arg-beta-naphthylamide | pH 8.00, 25°C | Escherichia coli | |
| 4850 | - |
benzoyl-Arg-beta-naphthylamide | pH 8.00, 25°C | Escherichia coli | |
| 5496 | - |
benzoyl-Arg-4-nitroanilide | pH 7.10, 25°C | Escherichia coli | |
| 5500 | - |
benzoyl-Arg-4-nitroanilide | pH 7.10, 25°C | Escherichia coli | |
| 6850 | - |
benzoyl-Arg-4-nitroanilide | pH 8.00, 25°C | Escherichia coli | |
| 6852 | - |
benzoyl-Arg-4-nitroanilide | pH 8.00, 25°C | Escherichia coli | |
| 11290 | - |
benzoyl-Arg-4-nitroanilide | pH 8.03, 25°C | Escherichia coli | |
| 11300 | - |
benzoyl-Arg-4-nitroanilide | pH 8.03, 25°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | - |
Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
benzoyl-Arg-4-nitroanilide | pH 8.10, 25°C | Escherichia coli | |
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