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Literature summary for 3.4.21.83 extracted from

  • Tsuru, D.; Yoshimoto, T.
    Oligopeptidase B: protease II from Escherichia coli (1994), Methods Enzymol., 244, 201-215.
    View publication on PubMed
Search for more literature for 3.4.21.83

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Escherichia coli
DFP
-
 Escherichia coli
additional information several natural trypsin inhibitors Escherichia coli
tosyl-Leu chloromethyl ketone
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
0.25
-
 benzoyl-Arg 2-naphthylamide
-
 Escherichia coli
0.48
-
 benzoyl-Arg ethyl ester
-
 Escherichia coli
0.5
-
 benzoyl-Arg 4-nitroanilide
-
 Escherichia coli
0.92
-
 Benzoyl-Lys 2-naphthylamide
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 Escherichia coli JM83 harboring pPR0II-12
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Benzoyl-Lys 2-naphthylamide + H2O
-
 Escherichia coli Benzoyl-Lys + 2-naphthylamine
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, pH 8.0, 50% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.2 Escherichia coli
8
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 stable Escherichia coli