Protein Variants | Comment | Organism |
---|---|---|
S195A/T213L/F228H | i.e. streptoligase, catalyzes peptide ligation efficiently. Ligation proceeds via an acyl-enzyme intermediate involving H57. Mutant exhibits half-life for unfolding of 16.3 min at 55°C in the absence of stabilizing substrates | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Sc-AAPF-p-nitroanilide + H2O | - |
Streptomyces griseus | ? | - |
? | |
Sc-AAPF-SBn + FAASF-NH2 | - |
Streptomyces griseus | ? | ligation to nonapeptide by mutant S195A/T213L/F228H | ? | |
Sc-AAPF-SBn + H2O | - |
Streptomyces griseus | ? | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ratio of ligation to hydrolysis by mutant S195A/T213L/F228H decreases with increase in temperature from 30°C to 60°C | Streptomyces griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
mutant S195A/T213L/F228H, half-life for unfolding of 16.3 min in the absence of stabilizing substrates | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
hydrolysis reaction | Streptomyces griseus |
8.8 | 9.2 | ligation reaction of mutant S195A/T213L/F228H | Streptomyces griseus |