Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.79 extracted from

  • Oberoi, P.; Jabulowsky, R.A.; Baehr-Mahmud, H.; Wels, W.S.
    EGFR-targeted granzyme B expressed in NK cells enhances natural cytotoxicity and mediates specific killing of tumor cells (2013), PLoS ONE, 8, e61267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.79

Cloned(Commentary)

Cloned (Comment) Organism
ectopic expression of granzyme B-epidermal growth factor receptor peptide ligand transforming growth factor alpha chimeric enzyme GrB-TGFalpha in natural killer cells from a lentiviral vector, the fusion enzyme shows enhanced natural cytotoxicity and increased specific killing of tumor cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of a recombinant chimeric enzyme by fusion of the pre-pro-granzyme B to the epidermal growth factor receptor peptide ligand transforming growth factor alpha. Activation of the genetically modified natural killer cells by cognate target cells resulted in the release of chimeric enzyme GrB-TGFalpha together with endogenous granzymes and perforin, which augments the effector cells natural cytotoxicity against NK-sensitive tumor cells. The chimeric enzyme GrB-TGFalpha is released into the extracellular space upon induction of degranulation with phspbol-12-myristate-13-acetate and ionomycin. The secreted GrB-TGFalpha chimeric enzyme displays specific binding to EGFR-overexpressing tumor cells, enzymatic activity, and selective target cell killing in the presence of an endosomolytic activity. Release of GrB-TGFalpha protein from NKL/GrB-TGFalpha cells upon activation-induced degranulation Homo sapiens
S183A inactive mutant Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, recombinnat chimeric enzyme GrB-TGFalpha, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P10144
-
-

Source Tissue

Source Tissue Comment Organism Textmining
natural killer cell
-
 Homo sapiens
-

Subunits

Subunits Comment Organism
? 1 * 40000, recombinnat chimeric enzyme GrB-TGFalpha, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
granzyme B
-
 Homo sapiens
GrB
-
 Homo sapiens
pro-apoptotic serine protease
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function natural killer cells exhibit cell killing activity that primarily mediated by the pro-apoptotic serine protease granzyme B, which enters targets cells with the help of the pore-forming protein perforin Homo sapiens