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Literature summary for 3.4.21.79 extracted from

  • Hou, Q.; Zhao, T.; Zhang, H.; Lu, H.; Zhang, Q.; Sun, L.; Fan, Z.
    Granzyme H induces apoptosis of target tumor cells characterized by DNA fragmentation and Bid-dependent mitochondrial damage (2008), Mol. Immunol., 45, 1044-1055.
    View publication on PubMed
Search for more literature for 3.4.21.79

Application

Application Comment Organism
medicine can induce rapid apoptosis of target cells, which is dependent on caspase activation and mitochondrial damage. GzmH-induced death is characterized by phosphatidylserine externalization, nuclear condensation, DNA fragmentation, caspase activation and cytochrome c release. GzmH may play an essential role in caspase-dependent pathogen clearance in the innate immunity that may complement the proapoptotic function of GzmB in human natural killer cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
active GzmH and mutant S182A with a C-terminal 6 x His-tag coding sequence subcloned into vector pPICZalpha and expressed in Pichia pastoris. Jurkat or HeLa cells loaded with GzmH Homo sapiens

Protein Variants

Protein Variants Comment Organism
S182A inactive Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 recombinant GzmH Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
active GzmH and mutant S182A purified by Ni-affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
BID + H2O cleaved in a dose-dependent fashion Homo sapiens truncated BID + ?
-
 ?
inhibitor of caspase-activated DNase + H2O cleaved in a dose-dependent fashion Homo sapiens caspase-activated DNase
-
 ?
pro-caspase 3 + H2O
-
 Homo sapiens p17 + ?
-
 ?
succinyl-Phe-Leu-Phe-p-nitroanilide + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Granzyme H
-
 Homo sapiens
GzmH
-
 Homo sapiens