Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | treating cells with the Hsp90 inhibitor 17-allylaminogeldanamycin does not inhibit GzmA-induced reactive oxygen species, cell death, or SET cleavage and in fact enhances each of these downstream events | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
K562 cells treated with perforin and recombinant human GzmA | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S176A | mutated active site, does not cleave protein NDUFS3 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | GzmA-mediated reactive oxygen species production and cell death are impaired in pseudo rho0 cells depleted of mitochondrial DNA. Expression of the K56A-NDUFS3 cleavage site mutant in 293T and K562 cells inhibits GzmA-mediated reactive oxygen species production and cell death | Homo sapiens | |
rotenone | the complex I inhibitor hinders GzmA-mediated reactive oxygen species production and cell death | Homo sapiens | |
valinomycin | pretreatment of K562 cells with 16 nmol valinomycin inhibits GzmA and perforin-induced reactive oxygen species generation | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein NDUFS3 + H2O | Homo sapiens | GzmA cleavage of native Ndufs3 disrupts complex I electron transport | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein NDUFS3 + H2O | GzmA cleavage of native Ndufs3 disrupts complex I electron transport | Homo sapiens | ? | - |
? | |
protein NDUFS3 + H2O | GzmA accesses the mitochondrial matrix to cleave the complex I protein NDUFS3, an iron-sulfur subunit of the NADH:ubiquinone oxidoreductase complex I, after Lys56 to interfere with NADH oxidation and generate superoxide anions. NDUFS3 is cleaved in cells treated with GzmA and perforin in a dose-dependent manner. Human GzmA cleaves both human and mouse NDUFS3. GzmA induces caspase-independent increased reactive oxygen species | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gzma | - |
Homo sapiens |