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Literature summary for 3.4.21.77 extracted from

  • Mattsson, J.M.; Ravela, S.; Hekim, C.; Jonsson, M.; Malm, J.; Naervaenen, A.; Stenman, U.H.; Koistinen, H.
    Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity (2014), PLoS ONE, 9, e107819.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.77

Activating Compound

Activating Compound Comment Organism Structure
CVAYCIEHHCWTC with disulfide bridges between cysteines 1-13 and 5-10, stimulates the activity of the enzyme towards small peptide substrates, and also enhances the proteolytic activity towards protein substrates Homo sapiens
CVFAHNYDYLVC stimulates the activity of the enzyme towards small peptide substrates, and also enhances the proteolytic activity towards protein substrates. No activation of the enzyme by the inactive derivative of peptide B2-NH2, CVFAHNADALVC, with a disulfide bridge between cysteines 1-12 Homo sapiens
additional information nidogen-1, galectin-3 or their fragments produced by the enzyme do not have any effect on endothelial cell tube formation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.58
-
 4-morpholinecarbonyl-HSSKLQ-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
5.72
-
 MeO-Suc-Arg-Pro-Tyr-4-nitroanilide pH 7.4, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
semenogelin I + H2O Homo sapiens from human seminal fluid ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P07288
-
-

Source Tissue

Source Tissue Comment Organism Textmining
prostate
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-morpholinecarbonyl-HSSKLQ-7-amido-4-methylcoumarin + H2O a fluorogenic enzyme substrate Homo sapiens ?
-
 ?
Fibronectin + H2O
-
 Homo sapiens ?
-
 ?
Galectin-3 + H2O
-
 Homo sapiens ?
-
 ?
IGFBP-3 + H2O
-
 Homo sapiens ?
-
 ?
MeO-Suc-Arg-Pro-Tyr-4-nitroanilide + H2O a chromogenic substrate Homo sapiens ?
-
 ?
additional information substrate specificity, overview Homo sapiens ?
-
 ?
nidogen-1 + H2O
-
 Homo sapiens ?
-
 ?
semenogelin I + H2O from human seminal fluid Homo sapiens ?
-
 ?
semenogelin II + H2O from human seminal fluid Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
kallikrein-related peptidase-3
-
 Homo sapiens
KLK3
-
 Homo sapiens
prostate-specific antigen
-
 Homo sapiens
PSA
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.19
-
 4-morpholinecarbonyl-HSSKLQ-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
0.75
-
 MeO-Suc-Arg-Pro-Tyr-4-nitroanilide pH 7.4, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function the main biological function of the enzyme is the liquefaction of the clot formed after ejaculation by cleavage of semenogelins I and II in seminal fluid. The enzyme also cleaves several other substrates, which may explain its putative functions in prostate cancer and its antiangiogenic activity. Analysis of the effect of PSA-degraded protein fragments on angiogenesis by the endothelial cell tube formation model, overview Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.122
-
 4-morpholinecarbonyl-HSSKLQ-7-amido-4-methylcoumarin pH 7.4, 37°C Homo sapiens
0.133
-
 MeO-Suc-Arg-Pro-Tyr-4-nitroanilide pH 7.4, 37°C Homo sapiens