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Literature summary for 3.4.21.76 extracted from

  • Fu, Z.; Thorpe, M.; Akula, S.; Chahal, G.; Hellman, L.T.
    Extended cleavage specificity of human neutrophil elastase, human proteinase 3, and their distant ortholog clawed frog PR3 - three elastases with similar primary but different extended specificities and stability (2018), Front. Immunol., 9, 2387 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.76

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged xPR-3 in HEK-293-EBNA cells using the episomal vector pCEPPu2 Xenopus laevis

General Stability

General Stability Organism
human proteinase 3 is stable to SDS Homo sapiens
the enzyme is instable in presence of SDS Xenopus laevis

Organism

Organism UniProt Comment Textmining
Homo sapiens P24158
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged xPR-3 from HEK-293 EBNA and activation and tag cleavage via enterokinase Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil from peripheral blood Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GLLASLGL + H2O
-
 Homo sapiens GLLA + Ser + LGL
-
 ?
GLLFSLGL + H2O
-
 Homo sapiens GLLF + Ser + LGL
-
 ?
GLLISLGL + H2O
-
 Homo sapiens GLLI + Ser + LGL
-
 ?
GLLVALGL + H2O
-
 Xenopus laevis GLLV + Ala + LGL
-
 ?
GLLVALGL + H2O
-
 Homo sapiens GLLV + Ala + LGL
-
 ?
GLLVDLGL + H2O
-
 Xenopus laevis GLLV + Asp + LGL
-
 ?
GLLVDLGL + H2O
-
 Homo sapiens GLLV + Asp + LGL
-
 ?
GLLVMLGL + H2O
-
 Xenopus laevis GLLV + Met + LGL
-
 ?
GLLVMLGL + H2O
-
 Homo sapiens GLLV + Met + LGL
-
 ?
GLLVRLGL + H2O
-
 Xenopus laevis GLLV + Arg + LGL
-
 ?
GLLVRLGL + H2O
-
 Homo sapiens GLLV + Arg + LGL
-
 ?
GLLVSLGL + H2O
-
 Xenopus laevis GLLV + Ser + LGL
-
 ?
GLLVSLGL + H2O
-
 Homo sapiens GLLV + Ser + LGL
-
 ?
GLLVWLGL + H2O
-
 Xenopus laevis GLLV + Trp + LGL
-
 ?
GLLVWLGL + H2O
-
 Homo sapiens GLLV + Trp + LGL
-
 ?
GLLWSLGL + H2O
-
 Homo sapiens GLLW + Ser + LGL
-
 ?
GRGVAGGRG + H2O
-
 Xenopus laevis GRGV + Ala + GGRG
-
 ?
GRGVSGGRG + H2O
-
 Xenopus laevis GRGV + Ser + GGRG
-
 ?
GRGVVVGRG + H2O
-
 Xenopus laevis GRGV + Val + Val + GRG
-
 ?
MLDAMGSL + H2O
-
 Homo sapiens ?
-
 ?
MLDTMGSL + H2O
-
 Homo sapiens ?
-
 ?
additional information analysis of substrate and cleavage specificity of hPR-3 using elastase substrates, having Val, Ala, and Ile in the P1 position, determination of the extended cleavage specificity, overview. hPR-3 shows good activity against the Val substrate, lower activity on the Ala substrate, and no activity on the other substrates, including one with an Ile in the P1 position Homo sapiens ?
-
 ?
additional information analysis of substrate and cleavage specificity of xPR-3 using elastase substrates, having Val, Ala, and Ile in the P1 position, determination of the extended cleavage specificity, overview. Xenopus PR-3 shows the best activity against the Ala substrate, lower activity on the Val, and similarly to human PR-3 no activity against the Ile substrate. MLDAMGSL and MLDTMGSL are poor substrates Xenopus laevis ?
-
 ?
VLLASEVL + H2O
-
 Xenopus laevis VLLA + SEVL
-
 ?
VLLFSEVL + H2O
-
 Xenopus laevis VLLF + SEVL
-
 ?
VLLISEVL + H2O
-
 Xenopus laevis VLLI + SEVL
-
 ?
VLLVSEVL + 3 H2O
-
 Homo sapiens VLLV + Ser + Glu + VL
-
 ?
VLLVSEVL + H2O
-
 Xenopus laevis VLLV + SEVL
-
 ?

Synonyms

Synonyms Comment Organism
hPR-3
-
 Homo sapiens
human PR3
-
 Homo sapiens
proteinase 3
-
 Xenopus laevis
proteinase 3
-
 Homo sapiens
Xenopus PR3
-
 Xenopus laevis
xPR-3
-
 Xenopus laevis

General Information

General Information Comment Organism
evolution presence of an elastase in Xenopus closely related to hPR-3 indicates a relatively early appearance of these enzymes during vertebrate evolution, sequence comparisons and phylogenetic analysis of the hematopoietic serine proteases, overview Xenopus laevis
evolution presence of an elastase in Xenopus closely related to hPR-3 indicates a relatively early appearance of these enzymes during vertebrate evolution, sequence comparisons and phylogenetic analysis of the hematopoietic serine proteases, overview Homo sapiens