Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.76 extracted from

  • Wysocka, M.; Lesner, A.; Majkowska, G.; Legowska, A.; Guzow, K.; Rolka, K.; Wiczk, W.
    The new fluorogenic substrates of neutrophil proteinase 3 optimized in prime site region (2010), Anal. Biochem., 399, 196-201.
    View publication on PubMed
Search for more literature for 3.4.21.76

Application

Application Comment Organism
additional information design of potent and highly specific substrates of proteinase 3 and other proteinases optimized in the prime site region Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0174
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gln-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0251
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asn-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0253
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Glu-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0278
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ser-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0281
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asp-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0314
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzamide) in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0321
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Thr-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0357
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-His-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0642
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Arg-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.0729
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Lys-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.1242
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gly-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.1731
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ala-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.1823
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Pro-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.2436
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Leu-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.2763
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ile-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.3124
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Val-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.9823
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Tyr-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.126
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Phe-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.274
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Trp-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzamide) + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + 5-amino-2-nitrobenzamide
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ala-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Ala-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Arg-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Arg-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asn-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Asn-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asp-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Asp-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gln-NH2 + H2O is hydrolyzed by PR3 within 20 min, yielding (5-amino-2-nitrobenzoyl)-Gln-NH2 and Abz-Tyr-Tyr-Abu fragments with retention times of 10.4 and 12.3 min, respectively Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Gln-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Glu-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Glu-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gly-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Gly-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-His-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-His-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ile-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Ile-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Leu-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Leu-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Lys-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Lys-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Phe-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Phe-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Pro-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Pro-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ser-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Ser-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Thr-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Thr-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Trp-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Trp-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Tyr-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Tyr-NH2
-
 ?
Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Val-NH2 + H2O
-
 Homo sapiens Abz-Tyr-Tyr-Abu + (5-amino-2-nitrobenzoyl)-Val-NH2
-
 ?
additional information most intense proteolysis of peptides with polar noncharged acid residues: Gln and Asn followed by Ser. Less active are peptides with negatively charged Glu and Asp. The presence of Lys and Arg gives substrates with susceptibility rates one order of magnitude lower Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PR3
-
 Homo sapiens
proteinase 3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Pro-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.3
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Phe-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.3
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Trp-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.6
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Tyr-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.8
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ile-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
2
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Leu-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
2.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ala-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
2.5
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Lys-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
2.6
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Arg-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
2.8
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Thr-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
3.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-His-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
3.2
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gly-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
3.3
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Glu-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
4.7
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Val-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
4.8
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asp-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
5.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ser-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
5.3
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asn-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
5.9
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzamide) in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
6.4
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gln-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Pro-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
0.9
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Trp-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Phe-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
1.6
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Tyr-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
6.5
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ile-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
7.2
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Leu-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
12.2
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ala-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
15.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Val-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
25.8
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gly-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
34.3
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Lys-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
41.1
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Arg-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
86.7
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-His-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
87.3
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Thr-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
138.2
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Glu-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
170.8
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asp-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
183.8
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Ser-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
188.9
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzamide) in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
201.2
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Asn-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens
274.9
-
 Abz-Tyr-Tyr-Abu-(5-amino-2-nitrobenzoyl)-Gln-NH2 in 0.1 M Tris-HCl buffer, pH 7.5, with 500 mM NaCl at 25°C Homo sapiens