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Literature summary for 3.4.21.76 extracted from

  • Korkmaz, B.; Moreau, T.; Gauthier, F.
    Neutrophil elastase, proteinase 3 and cathepsin G: physicochemical properties, activity and physiopathological functions (2008), Biochimie, 90, 227-242.
    View publication on PubMed
Search for more literature for 3.4.21.76

Application

Application Comment Organism
medicine responsible for eliminating intracellular pathogens and breaking down tissues at inflammatory sites. Possible molecular target for anti-inflammatory agents. Involved in degradation of ECM components and cleavage of inflammatory mediators, platlet activation, induction of endothelial cells and apoptosis, negative feedback regulation of granulopoiesis. Shows bactericidal properties. Is a target of auto-antibodies in Wegener’s granulomatosis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of recombinant PR3. Overall fold consists of two beta-barrel domains. PR3 structure includes a disaccharide unit covalently attached to Asn159. PR3 substrate binding sites at S4 to S3' Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha1-PI
-
 Homo sapiens
alpha2-Macroglobulin
-
 Homo sapiens
elafin
-
 Homo sapiens
MNEI
-
 Homo sapiens
additional information transcription of PR3 is downregulated upon granulocyte and monocyte maturation Homo sapiens
pre-elafin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface granulocytes and monocytes Homo sapiens 9986
-
membrane secretory vesicles and secondary granules Homo sapiens 16020
-
secondary granule
-
 Homo sapiens
-
-
secretory vesicle
-
 Homo sapiens 99503
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P24158
-
-

Source Tissue

Source Tissue Comment Organism Textmining
basophil
-
 Homo sapiens
-
epithelial cell inflamed oral epithelial cell Homo sapiens
-
granulocyte active PR3 stored in neutrophil azurophil granules Homo sapiens
-
monocyte
-
 Homo sapiens
-
additional information mRNA for PR3 is transcribed in the promyelocyte and promonocyte stages of myeloid differentiation. Present in mastocytes and no-hematopoietic cell Homo sapiens
-
neutrophil blood neutrophils from patients with active Wegener’s granulomatosis bear greater amounts of PR3 on their cell membranes than do neutrophils from healthy individuals Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-VADCRDRQ-EDDnp + H2O best synthetic substrate Homo sapiens Abz-VADCR + DRQ-EDDnp
-
 ?
Elastin + H2O
-
 Homo sapiens Hydrolyzed elastin
-
 ?
FRET + H2O
-
 Homo sapiens ?
-
 ?
IL-18 + H2O
-
 Homo sapiens ?
-
 ?
IL-1beta + H2O
-
 Homo sapiens ?
-
 ?
additional information substrate specificity for small hydrophobic residues at P1 position (Val, Cys, Ala, Met, Ser and Leu) Homo sapiens ?
-
 ?
NF-kappaB + H2O
-
 Homo sapiens ?
-
 ?
oxidized insulin B chain + H2O
-
 Homo sapiens ?
-
 ?
p21 + H2O
-
 Homo sapiens ?
-
 ?
protease-activated receptor-2 + H2O
-
 Homo sapiens ?
-
 ?
TNF-alpha + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? 29000-32000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
PR3
-
 Homo sapiens
proteinase 3
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 the mature PR3 has an inactive conformation in the acidic environment of the azurophilic granules, and becomes active upon translocation to a neutral pH environment Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens
-
-
 9.5