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Literature summary for 3.4.21.76 extracted from

  • Wiesner, O.; Litwiller, R.D.; Hummel, A.M.; Viss, M.A.; McDonald, C.J.; Jenne, D.E.; Fass, D.N.; Specks, U.
    Differences between human proteinase 3 and neutrophil elastase and their murine homologues are relevant for murine model experiments (2005), FEBS Lett., 579, 5305-5312.
    View publication on PubMed
Search for more literature for 3.4.21.76

Cloned(Commentary)

Cloned (Comment) Organism
expression in HMC-1 cells and 293 cells Homo sapiens
expression in HMC-1 cells and 293 cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
alpha1-antitrypsin MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 0.98 M Homo sapiens
alpha1-antitrypsin MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 0.74 M Mus musculus
Eglin c MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 117 M Homo sapiens
Eglin c MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 3.3 M Mus musculus
elafin MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 1.9 M Homo sapiens
elafin MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 0.99 M Mus musculus
SLPI MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, limited inhibition, more than 75% of activity remained in the presence of the highest concentration of inhibitor Homo sapiens
SLPI MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, limited inhibition, more than 75% of activity remained in the presence of the highest concentration of inhibitor Mus musculus
Val15-aprotinin MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, limited inhibition, more than 75% of activity remained in the presence of the highest concentration of inhibitor Homo sapiens
Val15-aprotinin MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 447 M Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
 MeOSuc-AIPM-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens
0.61
-
 Suc-AAA-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
1.1
-
 MeOSuc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
1.2
-
 MeOSuc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens
1.5
-
 MeOSuc-AIPM-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
2.5
-
 Suc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
3.5
-
 Suc-AAPL-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
3.7
-
 Suc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens P24158
-
-
Mus musculus Q61096
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
HMC-1 cell
-
 Homo sapiens
-
neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
MeOSuc-AAPV-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens MeOSuc-AAPV + 4-nitroaniline
-
 ?
MeOSuc-AAPV-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus MeOSuc-AAPV + 4-nitroaniline
-
 ?
MeOSuc-AIPM-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens MeOSuc-AIPM + 4-nitroaniline
-
 ?
MeOSuc-AIPM-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus MeOSuc-AIPM + 4-nitroaniline
-
 ?
oxidized insulin B chain + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens ?
-
 ?
oxidized insulin B chain + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus ?
-
 ?
Suc-AAA-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus Suc-AAA + 4-nitroaniline
-
 ?
Suc-AAA-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0, very low activity Homo sapiens Suc-AAA + 4-nitroaniline
-
 ?
Suc-AAPL-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus Suc-AAPL + 4-nitroaniline
-
 ?
Suc-AAPL-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0, very low activity Homo sapiens Suc-AAPL + 4-nitroaniline
-
 ?
Suc-AAPV-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens Suc-AAPV + 4-nitroaniline
-
 ?
Suc-AAPV-4-nitroanilide + H2O 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus Suc-AAPV + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
PR3
-
 Homo sapiens
PR3
-
 Mus musculus
proteinase 3
-
 Homo sapiens
proteinase 3
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.031
-
 Suc-AAA-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
0.16
-
 Suc-AAPL-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
0.26
-
 MeOSuc-AIPM-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens
0.37
-
 Suc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens
0.59
-
 MeOSuc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Homo sapiens
6.2
-
 MeOSuc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
7.2
-
 MeOSuc-AIPM-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus
11.2
-
 Suc-AAPV-4-nitroanilide 52 mM NaCl, 0.5% Triton X-100 (w/v), 10% dimethylformamide (v/v), pH 8.0 Mus musculus

pI Value

Organism Comment pI Value Maximum pI Value
Mus musculus calculated from amino acid sequence
-
 6.7
Homo sapiens calculated from amino acid sequence
-
 7.7

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
740
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 0.74 M Mus musculus alpha1-antitrypsin
980
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 0.98 M Homo sapiens alpha1-antitrypsin
990
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 0.99 M Mus musculus elafin
1900
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 1.9 M Homo sapiens elafin
3300
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 3.3 M Mus musculus Eglin c
117000
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 117 M Homo sapiens Eglin c
447000
-
 MeOSuc-AAPV-4-nitroanilide as substrate, 1% Triton X-100 (w/v), 20% dimethylformamide (v/v), pH 8.0, IC50 = 447 M Mus musculus Val15-aprotinin