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Literature summary for 3.4.21.75 extracted from

  • Hada, K.; Isshiki, K.; Matsuda, S.; Yuasa, K.; Tsuji, A.
    Engineering of alpha1-antitrypsin variants with improved specificity for the proprotein convertase furin using site-directed random mutagenesis (2013), Protein Eng. Des. Sel., 26, 123-131.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of the enzyme in HEK-293 cells, co-expression of inhibitor mutant alpha1-antitrypsin M352R Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha1-antitrypsin
-
Homo sapiens
alpha1-antitrypsin M352R i.e. alpha1-PDX. Engineering of alpha1-antitrypsin variants, containing Arg at the P1 site within the reactive site loop, with improved specificity for the proprotein convertase furin using site-directed random mutagenesis, screening, overview. The engineered a1-antitrypsin variant carrying the RXXR consensus motif for furin within its reactive site loop. Furin-mediated maturation of bone morphogenetic protein-4 is completely inhibited by ectopic expression of the AVNR variant Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-bone morphogenetic protein-4 + H2O Homo sapiens
-
mature bone morphogenetic protein-4 + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P09958
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-bone morphogenetic protein-4 + H2O
-
Homo sapiens mature bone morphogenetic protein-4 + ?
-
?

Synonyms

Synonyms Comment Organism
Proprotein convertase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme is a member of the subtilisin-like proprotein convertase family Homo sapiens
physiological function furin mediates maturation of bone morphogenetic protein-4 Homo sapiens