Literature summary for 3.4.21.75 extracted from

Kuester, M.; Becker, G.L.; Hardes, K.; Lindberg, I.; Steinmetzer, T.; Than, M.E.
Purification of the proprotein convertase furin by affinity chromatography based on PC-specific inhibitors (2011), Biol. Chem., 392, 973-981.

Search for more literature for 3.4.21.75

Inhibitors

Inhibitors	Comment	Organism
acetyl-Val-Arg-4-amidinobenzylamide	-	Mus musculus
biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Pro-Arg-4-amidinobenzylamide	-	Mus musculus
biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Thr-Arg-4-amidinobenzylamide	-	Mus musculus
biotin-(8-(amino)-3,6-dioxa-octanoyl)3-Arg-Pro-Arg-4-amidinobenzylamide	-	Mus musculus
biotin-8-(amino)-3,6-dioxa-octanoyl-Arg-Pro-Arg-4-amidinobenzylamide	-	Mus musculus
biotin-8-(amino)-3,6-dioxa-octanoyl-Val-Arg-4-amidinobenzylamide	-	Mus musculus
phenylacetyl-Arg-Pro-Arg-4-amidinobenzylamide	-	Mus musculus
phenylacetyl-Arg-Thr-Arg-4-amidinobenzylamide	-	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification protocol combining the heterologous expression of furin from CHO cells with an affinity step that efficiently extracts only active furin from the conditioned medium by using furin-specific inhibitor moieties as bait. The best affinity tag used, biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Pro-Arg-4-4-amidinobenzylamide coupled to streptavidin-Sepharose beads, is used in a three-step chromatographic protocol and routinely results in a high yield of a homogeneous furin preparation with a specific activity of about 60 units/mg protein	Mus musculus

Purification (Comment)

Organism

purification protocol combining the heterologous expression of furin from CHO cells with an affinity step that efficiently extracts only active furin from the conditioned medium by using furin-specific inhibitor moieties as bait. The best affinity tag used, biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Pro-Arg-4-4-amidinobenzylamide coupled to streptavidin-Sepharose beads, is used in a three-step chromatographic protocol and routinely results in a high yield of a homogeneous furin preparation with a specific activity of about 60 units/mg protein

Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O	-	Mus musculus	Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.000006	-	phenylacetyl-Arg-Thr-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.000009	-	biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Thr-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.0000151	-	biotin-(8-(amino)-3,6-dioxa-octanoyl)3-Arg-Pro-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.0000367	-	phenylacetyl-Arg-Pro-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.0000383	-	biotin-8-(amino)-3,6-dioxa-octanoyl-Arg-Pro-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.0000574	-	biotin-(8-(amino)-3,6-dioxa-octanoyl)2-Arg-Pro-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.00239	-	acetyl-Val-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus
0.0042	-	biotin-8-(amino)-3,6-dioxa-octanoyl-Val-Arg-4-amidinobenzylamide	pH 7.0, 37°C	Mus musculus