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Literature summary for 3.4.21.75 extracted from

  • Izidoro, M.A.; Gouvea, I.E.; Santos, J.A.; Assis, D.M.; Oliveira, V.; Judice, W.A.; Juliano, M.A.; Lindberg, I.; Juliano, L.
    A study of human furin specificity using synthetic peptides derived from natural substrates, and effects of potassium ions (2009), Arch. Biochem. Biophys., 487, 105-114.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.75

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00007
-
 hBMP-2 precursor protein first cleavage site, pH 7.0, 37°C Homo sapiens
0.0001
-
 pro-hTGF-beta2 protein pH 7.0, 37°C Homo sapiens
0.0001
-
 pro-hTGF-best1 protein pH 7.0, 37°C Homo sapiens
0.00011
-
 pro-MT-MMP 11 protein pH 7.0, 37°C Homo sapiens
0.00011
-
 pro-MT-MMP 3 protein pH 7.0, 37°C Homo sapiens
0.00014
-
 pro-hTGF-beta3 protein pH 7.0, 37°C Homo sapiens
0.00015
-
 pro-MT-MMP 1 protein pH 7.0, 37°C Homo sapiens
0.0002
-
 pro-hADAM-15 protein pH 7.0, 37°C Homo sapiens
0.00037
-
 pro-MT-MMP 6 protein pH 7.0, 37°C Homo sapiens
0.0004
-
 pro-hADAM-TS 6 protein pH 7.0, 37°C Homo sapiens
0.00045
-
 pro-MT-MMP 4 protein pH 7.0, 37°C Homo sapiens
0.00055
-
 pro-hADAM-TS 4 protein pH 7.0, 37°C Homo sapiens
0.00064
-
 proaerolysin pH 7.0, 37°C Homo sapiens
0.0013
-
 hBMP-2 precursor protein second cleavage site, pH 7.0, 37°C Homo sapiens
0.0014
-
 hBMP-4 precursor protein second cleavage site, pH 7.0, 37°C Homo sapiens
0.0015
-
 hBMP-4 precursor protein first cleavage site, pH 7.0, 37°C Homo sapiens
0.00194
-
 acetyl-RVRR-4-methylcoumarin 7-amide pH 7.0, 37°C Homo sapiens
0.0022
-
 pro-hTGF-beta4 protein pH 7.0, 37°C Homo sapiens
0.0026
-
 Shiga toxin pH 7.0, 37°C Homo sapiens
0.0067
-
 pro-hADAMTS-23 protein pH 7.0, 37°C Homo sapiens
0.0091
-
 pro-hADAMTS-17 protein pH 7.0, 37°C Homo sapiens
0.0118
-
 Pseudomonas toxin pH 7.0, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GIRRKRSVSHQ-EDDnp + H2O
-
 Homo sapiens Abz-GIRRKR + SVSHQ-EDDnp
-
 ?
Abz-SGRSRRAIDLQEDDnp + H2O
-
 Homo sapiens Abz-SGRSRR + AIDLQEDDnp
-
 ?
Abz-SKRSRRSVSVQ-EDDnp + H2O
-
 Homo sapiens Abz-SKRSRR + SVSVQ-EDDnp
-
 ?
acetyl-RVRR-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens acetyl-RVRR + 7-amino-4-methylcoumarin
-
 ?
hBMP-2 precursor protein + H2O cleavage sites are HKREKR-/-QAKH and HVRISR-/-SLHQ Homo sapiens ?
-
 ?
hBMP-4 precursor protein + H2O cleavage sites are RRRAKR-/-SPKH and HVRISR-/-SLPQ Homo sapiens ?
-
 ?
additional information furin is a proprotein convertase that requires the cleavage sequence R-X-K/R-R, clear interdependence of furin subsites, substrate specificity with synthetic peptide substrates, overview Homo sapiens ?
-
 ?
pro-hADAM-15 protein + H2O cleavage site is HIRRRR-/-DVVT Homo sapiens ?
-
 ?
pro-hADAM-TS 4 protein + H2O cleavage site is RPRRAKR-/-FASL Homo sapiens ?
-
 ?
pro-hADAM-TS 6 protein + H2O cleavage site is HHRQRR-/-SVSI Homo sapiens ?
-
 ?
pro-hADAMTS-17 protein + H2O cleavage site is HVRKRR-/-ADPD Homo sapiens ?
-
 ?
pro-hADAMTS-23 protein + H2O cleavage site is LKRRKR-/-AVNP Homo sapiens ?
-
 ?
pro-hTGF-best1 protein + H2O
-
 Homo sapiens ?
-
 ?
pro-hTGF-beta1 protein + H2O cleavage site is NRRKKR-/-ALDA Homo sapiens ?
-
 ?
pro-hTGF-beta2 protein + H2O cleavage site is GQRKKR-/-ALDT Homo sapiens ?
-
 ?
pro-hTGF-beta3 protein + H2O cleavage site is SSRHRR-/-ALDT Homo sapiens ?
-
 ?
pro-hTGF-beta4 protein + H2O cleavage site is RSRGRR-/-FSQS Homo sapiens ?
-
 ?
pro-MT-MMP 1 protein + H2O cleavage site is NVRRKR-/-YALT Homo sapiens ?
-
 ?
pro-MT-MMP 11 protein + H2O cleavage site is RHRQKR-/-FVLS Homo sapiens ?
-
 ?
pro-MT-MMP 3 protein + H2O cleavage site is RNRQKR-/-FVLS Homo sapiens ?
-
 ?
pro-MT-MMP 4 protein + H2O cleavage site is QSRRRR-/-QTPP Homo sapiens ?
-
 ?
pro-MT-MMP 6 protein + H2O cleavage site is VRRRRR-/-YALS Homo sapiens ?
-
 ?
proaerolysin + H2O cleavage site is KVRRAR-/-SVDG Homo sapiens ?
-
 ?
Pseudomonas toxin + H2O cleavage site is RHRQPR-/-GWEQ Homo sapiens ?
-
 ?
Shiga toxin + H2O cleavage site is ASRVAR-/-MASD Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
More furin belongs to MEROPS clan SB, family S8 Homo sapiens
Proprotein convertase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 pro-MT-MMP 3 protein pH 7.0, 37°C Homo sapiens
0.54
-
 Pseudomonas toxin pH 7.0, 37°C Homo sapiens
0.7
-
 acetyl-RVRR-4-methylcoumarin 7-amide pH 7.0, 37°C Homo sapiens
0.84
-
 pro-MT-MMP 6 protein pH 7.0, 37°C Homo sapiens
1
-
 pro-hADAMTS-23 protein pH 7.0, 37°C Homo sapiens
1
-
 pro-hADAMTS-17 protein pH 7.0, 37°C Homo sapiens
1.2
-
 pro-hTGF-beta4 protein pH 7.0, 37°C Homo sapiens
1.3
-
 pro-MT-MMP 11 protein pH 7.0, 37°C Homo sapiens
1.5
-
 pro-hTGF-beta1 protein pH 7.0, 37°C Homo sapiens
2.4
-
 pro-MT-MMP 1 protein pH 7.0, 37°C Homo sapiens
3
-
 hBMP-2 precursor protein second cleavage site, pH 7.0, 37°C Homo sapiens
3.4
-
 pro-hTGF-beta2 protein pH 7.0, 37°C Homo sapiens
3.6
-
 hBMP-2 precursor protein first cleavage site, pH 7.0, 37°C Homo sapiens
3.6
-
 hBMP-4 precursor protein second cleavage site, pH 7.0, 37°C Homo sapiens
4.5
-
 pro-hADAM-TS 4 protein pH 7.0, 37°C Homo sapiens
5.5
-
 pro-hADAM-15 protein pH 7.0, 37°C Homo sapiens
5.5
-
 pro-MT-MMP 4 protein pH 7.0, 37°C Homo sapiens
5.6
-
 hBMP-4 precursor protein first cleavage site, pH 7.0, 37°C Homo sapiens
8.4
-
 pro-hTGF-beta3 protein pH 7.0, 37°C Homo sapiens
12.2
-
 proaerolysin pH 7.0, 37°C Homo sapiens
16
-
 Shiga toxin pH 7.0, 37°C Homo sapiens
40
-
 pro-hADAM-TS 6 protein pH 7.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH-profiles of furin activity in the presence and absence of salts, e.g. KCl. The pK1 values for the pH-profiles of hydrolysis of all four substrates in the presence of KCl are lower than those in its absence Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
45
-
 Pseudomonas toxin pH 7.0, 37°C Homo sapiens
109
-
 pro-hADAMTS-17 protein pH 7.0, 37°C Homo sapiens
143
-
 pro-hADAMTS-23 protein pH 7.0, 37°C Homo sapiens
361
-
 acetyl-RVRR-4-methylcoumarin 7-amide pH 7.0, 37°C Homo sapiens
534
-
 pro-hTGF-beta4 protein pH 7.0, 37°C Homo sapiens
2240
-
 pro-MT-MMP 6 protein pH 7.0, 37°C Homo sapiens
2331
-
 hBMP-2 precursor protein second cleavage site, pH 7.0, 37°C Homo sapiens
2649
-
 hBMP-4 precursor protein second cleavage site, pH 7.0, 37°C Homo sapiens
3700
-
 hBMP-4 precursor protein first cleavage site, pH 7.0, 37°C Homo sapiens
4545
-
 pro-MT-MMP 3 protein pH 7.0, 37°C Homo sapiens
6134
-
 Shiga toxin pH 7.0, 37°C Homo sapiens
8127
-
 pro-hADAM-TS 4 protein pH 7.0, 37°C Homo sapiens
11450
-
 pro-MT-MMP 11 protein pH 7.0, 37°C Homo sapiens
12670
-
 pro-MT-MMP 4 protein pH 7.0, 37°C Homo sapiens
14700
-
 pro-hTGF-beta1 protein pH 7.0, 37°C Homo sapiens
15800
-
 pro-MT-MMP 1 protein pH 7.0, 37°C Homo sapiens
20670
-
 proaerolysin pH 7.0, 37°C Homo sapiens
27600
-
 pro-hADAM-15 protein pH 7.0, 37°C Homo sapiens
34200
-
 pro-hTGF-beta2 protein pH 7.0, 37°C Homo sapiens
50710
-
 hBMP-2 precursor protein first cleavage site, pH 7.0, 37°C Homo sapiens
60140
-
 pro-hTGF-beta3 protein pH 7.0, 37°C Homo sapiens
100000
-
 pro-hADAM-TS 6 protein pH 7.0, 37°C Homo sapiens