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Literature summary for 3.4.21.75 extracted from

  • Wanyiri, J.W.; OConnor, R.; Allison, G.; Kim, K.; Kane, A.; Qiu, J.; Plaut, A.G.; Ward, H.D.
    Proteolytic processing of the Cryptosporidium glycoprotein gp40/15 by human furin and by a parasite-derived furin-like protease activity (2007), Infect. Immun., 75, 184-192.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.75

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ parasite expresses a Ca2+ dependent, furin-like protease activity Cryptosporidium parvum

Application

Application Comment Organism
medicine furin-like protease activity may be involved in mediating host-parasite interactions. Inhibition of infection is due to an effect on the parasite-derived furin activity rather than the host cell furin Cryptosporidium parvum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Cryptosporidium parvum
4-(2-aminoethyl)benzenesulphonyl fluoride
-
 Cryptosporidium parvum
decanoyl-Arg-Val-Lys-Arg-chloromethylketone most inhibitory, inhibits Cryptosporidium parvum infection of HCT-8 cells in a dose-dependent manner, with ca. 50% inhibition occurring at a concentration of 0.01 mM Cryptosporidium parvum
EDTA 97% inhibition, Ca2+ can reverse the inhibitory effect Cryptosporidium parvum
EDTA completely inhibits Homo sapiens
EGTA
-
 Cryptosporidium parvum
Glu-Gly-Arg-chloromethylketone poor inhibitor Cryptosporidium parvum
leupeptin
-
 Cryptosporidium parvum
additional information aprotinin has no effect on furin-like protease activity Cryptosporidium parvum
N-tosyl-L-lysine chloromethyl ketone
-
 Cryptosporidium parvum
Phe-Pro-Arg-chloromethylketone poor inhibitor Cryptosporidium parvum
phenylmethylsulfonyl fluoride
-
 Cryptosporidium parvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01517
-
 t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin
-
 Cryptosporidium parvum

Organism

Organism UniProt Comment Textmining
Cryptosporidium parvum
-
-
-
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HCT-8 cell
-
 Homo sapiens
-
additional information lysate Cryptosporidium parvum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
gp40/15 + H2O cleaves recombinant Cryptosporidium parvum and Cryptosporidium hominis gp40/15. Putative furin cleavage site RSRR Homo sapiens gp40 + gp15
-
 ?
gp40/15 + H2O putative furin cleavage site RSRR Cryptosporidium parvum gp40 + gp15
-
 ?
gp40/15 subtype 1e + H2O RSRR sequence is replaced by ISKR, has an alternative furin cleavage site at KSISKR2 Homo sapiens gp40 + gp15
-
 ?
gp40/15 subtype 1e + H2O RSRR sequence is replaced by ISKR, has an alternative furin cleavage site at KSISKR2 Cryptosporidium parvum gp40 + gp15
-
 ?
additional information mutation in the RSRR cleavage site prevents processing of gp40/15 Homo sapiens ?
-
 ?
additional information mutation in the RSRR cleavage site prevents processing of gp40/15 Cryptosporidium parvum ?
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens ?
-
 ?
t-butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Cryptosporidium parvum ?
-
 ?

Synonyms

Synonyms Comment Organism
furin-like protease
-
 Cryptosporidium parvum
subtilisin-like proprotein convertase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Cryptosporidium parvum

pH Range

pH Minimum pH Maximum Comment Organism
4 8
-
 Cryptosporidium parvum