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Literature summary for 3.4.21.75 extracted from

  • Dufour, E.K.; Desilets, A.; Longpre, J.M.; Leduc, R.
    Stability of mutant serpin/furin complexes: dependence on pH and regulation at the deacylation step (2005), Protein Sci., 14, 303-315.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
alpha1-Aantichymotrypsin incorporation of furin recognition sequences within the reactive site loop of alpha1-antiprypsin leads to the production of furin inhibitors, construction of a series of alpha1-antichymotrypsin mutants by modifying the P7-P1 region of the reactive site loop Homo sapiens
alpha1-antitrypsin incorporation of furin recognition sequences within the reactive site loop of alpha1-antiprypsin leads to the production of furin inhibitors, construction of a series of alpha1-antitrypsin mutants by modifying the P7-P1 region of the reactive site loop Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information upon increase of the pH from 6.0 to 9.0, KM-decreases linearly up to pH 7.5 and then remains constant Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tert-butyloxycarbonyl-RVRR-4-methylcoumaryl-7-amide + H2O
-
Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information over the pH intercal 6.0 to 9.0 kcat occurs as a bell-shaped form with a maximum at pH 7.0 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
hydrolysis tert-butyloxycarbonyl-RVRR-4-methylcoumaryl-7-amide Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 70% of maximal activity, pH 8.0: about 65% of maximal activity Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information Ki-values of alpha1-antitrypsin and alpha1-antichymotrypsin mutants obtained by incorporation of furin recognition sequences within the reactive site loop of the molecule and modifying the P7-P1 region of the reactive site loop Homo sapiens