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Literature summary for 3.4.21.75 extracted from

  • Cieplik, M.; Klenk, H.D.; Garten, W.
    Identification and characterization of spodoptera frugiperda furin: a thermostable subtilisin-like endopeptidase (1998), Biol. Chem., 379, 1433-1440.
    View publication on PubMed
Search for more literature for 3.4.21.75

Cloned(Commentary)

Cloned (Comment) Organism
cDNA sequenced, cloned from a cDNA library of Sf9 cells, overexpressed from a baculovirus vector in Sf9 cells Spodoptera frugiperda
recombinant Bfurin expressed in Sf9 cells Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
chymostatin
-
 Bos taurus
chymostatin
-
 Spodoptera frugiperda
CuSO4
-
 Bos taurus
CuSO4
-
 Spodoptera frugiperda
EDTA
-
 Bos taurus
EDTA
-
 Spodoptera frugiperda
HgCl2
-
 Bos taurus
HgCl2
-
 Spodoptera frugiperda
iodoacetamide about 50% inhibition Bos taurus
iodoacetamide about 50% inhibition Spodoptera frugiperda
additional information largely unaffected by aprotinin, leupeptin, pepstatin A, [4-amidino-phenyl]-methansulfonylfluoride, (4-[2-aminoethyl]-benzolsulfonylfluoride), phosphoramidon, bestatin, phenylmethylsulfonylfluoride, and antipain Bos taurus
additional information largely unaffected by aprotinin, leupeptin, pepstatin A, [4-amidino-phenyl]-methansulfonylfluoride, (4-[2-aminoethyl]-benzolsulfonylfluoride), phosphoramidon, bestatin, phenylmethylsulfonylfluoride, and antipain Spodoptera frugiperda
ZnCl2
-
 Bos taurus
ZnCl2
-
 Spodoptera frugiperda

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus trans Golgi network Spodoptera frugiperda 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activated at concentrations below 0.01 mM Spodoptera frugiperda
Ca2+ activated between 0.1 and 10 mM, optimum at about 1 mM Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Spodoptera frugiperda plays an important role in posttranslatioal protein processing ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Spodoptera frugiperda Q26489
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FPV precursor molecule HA0 + H2O
-
 Bos taurus subunit HA1 + subunit HA2
-
 ?
FPV precursor molecule HA0 + H2O
-
 Spodoptera frugiperda subunit HA1 + subunit HA2
-
 ?
additional information LRR peptidyl-7-amido-4-methyl-coumarin is no substrate Bos taurus ?
-
 ?
additional information LRR peptidyl-7-amido-4-methyl-coumarin is no substrate Spodoptera frugiperda ?
-
 ?
additional information plays an important role in posttranslatioal protein processing Spodoptera frugiperda ?
-
 ?
peptidyl-7-amido-4-methyl-coumarin + H2O
-
 Bos taurus 7-amino-4-methyl-coumarin + peptide
-
 ?
peptidyl-7-amido-4-methyl-coumarin + H2O
-
 Spodoptera frugiperda 7-amino-4-methyl-coumarin + peptide
-
 ?
RTKR 4-methyl-coumarin 7-amide + H2O
-
 Bos taurus 7-amino-4-methyl-coumarin + RTKR
-
 ?
RTKR 4-methyl-coumarin 7-amide + H2O
-
 Spodoptera frugiperda 7-amino-4-methyl-coumarin + RTKR
-
 ?

Synonyms

Synonyms Comment Organism
Bfurin
-
 Bos taurus
Sfurin
-
 Spodoptera frugiperda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 rapidly inactivated by increasing temperatures above Bos taurus
60
-
 stable up to Spodoptera frugiperda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
 Bos taurus
7.5 9
-
 Spodoptera frugiperda

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Bos taurus
6 11
-
 Spodoptera frugiperda