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Literature summary for 3.4.21.74 extracted from
- Bhalternin: Functional and structural characterization of a new thrombin-like enzyme from Bothrops alternatus snake venom (2010), Toxicon, 55, 1365-1377.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | Bhaltenin may be of interest as a therapeutic agent in the treatment and prevention of thrombotic disorders | Bothrops alternatus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Bothrops alternatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzamidine | - |
Bothrops alternatus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bothrops alternatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
1 * 27000, SDS-PAGE | Bothrops alternatus |
| 31500 | - |
native PAGE | Bothrops alternatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrinogen + H2O | Bothrops alternatus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bothrops alternatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from venom by anion exchange chromatography, gel filtration, and benzamidine affinity chromatography | Bothrops alternatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Bothrops alternatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Albumin + H2O | bovine substrate | Bothrops alternatus | ? | - |
? | |
| azocasein + H2O | - |
Bothrops alternatus | ? | - |
? | |
| Fibrinogen + H2O | - |
Bothrops alternatus | ? | - |
? | |
| Fibrinogen + H2O | bovine substrate, the enzyme cleaves preferentially the Aalpha-chain, apparently not degrading the Bbeta and gamma-chains | Bothrops alternatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, SDS-PAGE | Bothrops alternatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bhalternin | - |
Bothrops alternatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Bothrops alternatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Bothrops alternatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is a thrombin-like serine protease | Bothrops alternatus |
| physiological function | the enzyme causes morphological alterations in heart, liver, lung and muscle of mice and causes blood clotting in vitro and defibrinogenation when intraperitoneally administered to mice, suggesting it to be a thrombin-like enzyme | Bothrops alternatus |
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