Cloned (Comment) | Organism |
---|---|
expression of the enzyme N-terminally fused separately to three fusion partners, NusA, GST, and TrxA, expression in Escherichia coli strain BL21-Gold (DE3). NusA is the most efficient fusion partner to improve the solubility of recombinant gloshedobin, subcloning in Escherichia coli strain JM109 | Gloydius shedaoensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by ethylenediaminetetraacetic acid, heparin, and hirudin. 10 mM 2-mercaptoethanol and DTT have poor effects on the recombinant enzyme activity | Gloydius shedaoensis | |
N-tosyl-L-phenylalanine chloromethyl ketone | strong inhibition | Gloydius shedaoensis | |
phenylmethylsulfonyl fluoride | strong inhibition | Gloydius shedaoensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Gloydius shedaoensis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, recombinant HusA-fusion gloshedobin, SDS-PAGE | Gloydius shedaoensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gloydius shedaoensis | P0C5B4 | - |
- |
Purification (Comment) | Organism |
---|---|
soluble recombinant NusA-fusion gloshedobin from Escherichia coli by hydrophobic interaction chromatograpy, two steps of anion exchange chromatography, and gel filtration | Gloydius shedaoensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Gloydius shedaoensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
506 | - |
purified recombinant NusA-fusion gloshedobin, pH 8.0, 40°C | Gloydius shedaoensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-Arg-4-nitroanilide + H2O | - |
Gloydius shedaoensis | benzoyl-Arg + 4-nitroaniline | - |
? | |
Fibrinogen + H2O | cleavage of the alpha-chain and the beta-chain, but not of the gamma-chain. The band of alpha chain of fibrinogen became invisible on the gel after 2 h of the reaction. Moreover, the beta chain of fibrinogen is fully digested after 24 h of the reaction | Gloydius shedaoensis | ? | - |
? | |
additional information | recombinant NusA-fusion gloshedobin shows both fibrinogen clotting and fibrinogenolytic activities | Gloydius shedaoensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, recombinant HusA-fusion gloshedobin, SDS-PAGE | Gloydius shedaoensis |
Synonyms | Comment | Organism |
---|---|---|
gloshedobin | - |
Gloydius shedaoensis |
venom gloshedobin | - |
Gloydius shedaoensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant NusA-fusion gloshedobin | Gloydius shedaoensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, recombinant NusA-fusion gloshedobin, profile, overview | Gloydius shedaoensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant NusA-fusion gloshedobin | Gloydius shedaoensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | activity range, recombinant NusA-fusion gloshedobin, profile, overview | Gloydius shedaoensis |
General Information | Comment | Organism |
---|---|---|
additional information | gloshedobin is a thrombin-like enzyme from the venom of Gloydius shedaoensis | Gloydius shedaoensis |