Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.74 extracted from

  • Jiang, X.; Xu, J.; Yang, Q.
    Soluble expression, purification, and characterization of Gloydius shedaoensis venom gloshedobin in Escherichia coli by using fusion partners (2010), Appl. Microbiol. Biotechnol., 85, 635-642.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the enzyme N-terminally fused separately to three fusion partners, NusA, GST, and TrxA, expression in Escherichia coli strain BL21-Gold (DE3). NusA is the most efficient fusion partner to improve the solubility of recombinant gloshedobin, subcloning in Escherichia coli strain JM109 Gloydius shedaoensis

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by ethylenediaminetetraacetic acid, heparin, and hirudin. 10 mM 2-mercaptoethanol and DTT have poor effects on the recombinant enzyme activity Gloydius shedaoensis
N-tosyl-L-phenylalanine chloromethyl ketone strong inhibition Gloydius shedaoensis
phenylmethylsulfonyl fluoride strong inhibition Gloydius shedaoensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Gloydius shedaoensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
x * 90000, recombinant HusA-fusion gloshedobin, SDS-PAGE Gloydius shedaoensis

Organism

Organism UniProt Comment Textmining
Gloydius shedaoensis P0C5B4
-
-

Purification (Commentary)

Purification (Comment) Organism
soluble recombinant NusA-fusion gloshedobin from Escherichia coli by hydrophobic interaction chromatograpy, two steps of anion exchange chromatography, and gel filtration Gloydius shedaoensis

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Gloydius shedaoensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
506
-
purified recombinant NusA-fusion gloshedobin, pH 8.0, 40°C Gloydius shedaoensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg-4-nitroanilide + H2O
-
Gloydius shedaoensis benzoyl-Arg + 4-nitroaniline
-
?
Fibrinogen + H2O cleavage of the alpha-chain and the beta-chain, but not of the gamma-chain. The band of alpha chain of fibrinogen became invisible on the gel after 2 h of the reaction. Moreover, the beta chain of fibrinogen is fully digested after 24 h of the reaction Gloydius shedaoensis ?
-
?
additional information recombinant NusA-fusion gloshedobin shows both fibrinogen clotting and fibrinogenolytic activities Gloydius shedaoensis ?
-
?

Subunits

Subunits Comment Organism
? x * 90000, recombinant HusA-fusion gloshedobin, SDS-PAGE Gloydius shedaoensis

Synonyms

Synonyms Comment Organism
gloshedobin
-
Gloydius shedaoensis
venom gloshedobin
-
Gloydius shedaoensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant NusA-fusion gloshedobin Gloydius shedaoensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range, recombinant NusA-fusion gloshedobin, profile, overview Gloydius shedaoensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant NusA-fusion gloshedobin Gloydius shedaoensis

pH Range

pH Minimum pH Maximum Comment Organism
6 10 activity range, recombinant NusA-fusion gloshedobin, profile, overview Gloydius shedaoensis

General Information

General Information Comment Organism
additional information gloshedobin is a thrombin-like enzyme from the venom of Gloydius shedaoensis Gloydius shedaoensis