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Literature summary for 3.4.21.73 extracted from
- Purification and characterization of rat urinary esterase A, a plasminogen activator (1983), J. Biol. Chem., 258, 4434-4439.
General Stability
| General Stability | Organism |
|---|---|
| Unstable to dilution | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-1-antitrypsin | - |
Rattus norvegicus | |
| antipain | - |
Rattus norvegicus |  |
| Aprotinin | - |
Rattus norvegicus | |
| benzamidine | - |
Rattus norvegicus | |
| D-Phe-Phe-ArgCH2Cl | - |
Rattus norvegicus | |
| leupeptin | - |
Rattus norvegicus | |
| Lima bean trypsin inhibitor | - |
Rattus norvegicus | |
| Pentamidine | - |
Rattus norvegicus | |
| phenylmethanesulfonyl fluoride | - |
Rattus norvegicus | |
| Soybean trypsin inhibitor | - |
Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-trifluoromethylcoumarin | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8550 | - |
x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
| 8850 | - |
x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
| 14200 | - |
x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
| 15800 | - |
x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
| 23000 | - |
x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
| 24500 | - |
x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| urine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.01 M sodium phosphate buffer, pH 7.0, 0.2 mg/ml enzyme concentration, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| kininogen + H2O | - |
Rattus norvegicus | kinin + ? | - |
? | |
| additional information | lyses fibrin clots containing plasminogen but not plasminogen-free fibrin | Rattus norvegicus | ? | - |
? | |
| N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-trifluoromethylcoumarin + H2O | - |
Rattus norvegicus | N-benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-trifluoromethylcoumarin | - |
? | |
| plasminogen + H2O | - |
Rattus norvegicus | plasmin + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 15800 + x * 14200 + x * 8850 + x * 8550, SDS-PAGE under reducing conditions, x * 24500 + x * 23000, SDS-PAGE under nonreducing conditions | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Urinary esterase A | - |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30-60 min, 72-82% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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