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Literature summary for 3.4.21.72 extracted from

  • Zhigis, L.S.; Kotelnikova, O.V.; Vikhrov, A.A.; Zinchenko, A.A.; Serova, O.V.; Zueva, V.S.; Razgulyaeva, O.A.; Gordeeva, E.A.; Melikhova, T.D.; Nokel, E.A.; Alliluev, A.P.; Drozhzhina, E.Y.; Rumsh, L.D.
    A new methodological approach to estimation of IgA1 and IgA2 content in human serum using recombinant IgA1 protease from Neisseria meningitidis (2015), Biotechnol. Lett., 37, 2289-2293 .
    View publication on PubMed

Application

Application Comment Organism
analysis the ability of IgA1 protease to cleave exclusively IgA1 without affecting IgA2 molecules allows a more economical and reliable method for determination of these antibody subclasses in human sera based on recombinant Neisseria meningitidis IgA1 protease. Estimation of IgA1 and IgA2 content in human serum and of IgA subclass levels and IgA1/IgA2 ratio using recombinant active and inactive forms of IgA1 protease from Neisseria meningitidis, method evaluation, overview Neisseria meningitidis serogroup B

Protein Variants

Protein Variants Comment Organism
S267A site-directed mutagenesis, catalytically inactive mutant Neisseria meningitidis serogroup B

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
-
Neisseria meningitidis serogroup B

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
immunglobulin A1 + H2O Neisseria meningitidis serogroup B IgA1 from human serum immunglobulin Fc + immunglobulin Fd
-
?
immunglobulin A1 + H2O Neisseria meningitidis serogroup B H44/76 IgA1 from human serum immunglobulin Fc + immunglobulin Fd
-
?

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis serogroup B
-
-
-
Neisseria meningitidis serogroup B H44/76
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
immunglobulin A1 + H2O IgA1 from human serum Neisseria meningitidis serogroup B immunglobulin Fc + immunglobulin Fd
-
?
immunglobulin A1 + H2O IgA1 from human serum Neisseria meningitidis serogroup B H44/76 immunglobulin Fc + immunglobulin Fd
-
?

Synonyms

Synonyms Comment Organism
IgA1 protease
-
Neisseria meningitidis serogroup B

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Neisseria meningitidis serogroup B

General Information

General Information Comment Organism
malfunction the IgA1/IgA2 ratio is 6.3 for the healthy volunteers and 12.8 for patients with meningitis. IgA2 for the patients with meningitis and the healthy volunteers are almost unchanged, 1:86 and 1:121, respectively Neisseria meningitidis serogroup B
physiological function ability of IgA1 protease to cleave exclusively IgA1 without affecting IgA2 molecules Neisseria meningitidis serogroup B