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Literature summary for 3.4.21.71 extracted from

  • Szilagyi, C.M.; Sarfati, P.; Pradayrol, L.; Morisset, J.
    Purification, characterization and substrate specificity of rat pancreatic elastase II (1995), Biochim. Biophys. Acta, 1251, 55-65.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA clone isolated Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin causes total inhibition Rattus norvegicus
diisopropyl fluorophosphate causes total inhibition Rattus norvegicus
additional information enzyme activity is not affected by TPCK nor by phosphoramidon Rattus norvegicus
N-alpha-tosyl-L-lysine-chloromethyl ketone most potent inhibitor Rattus norvegicus
phenylmethylsulfonyl fluoride 1 mM, 50% inhibition Rattus norvegicus
Soybean trypsin inhibitor most potent inhibitor Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.089
-
Somatostatin-14 pH 8.0, 25°C Rattus norvegicus
1.567
-
Suc-Ala-Ala-Pro-Met-p-nitroanilide pH 8.0, 25°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information increasing Ca2+ activity has no effect on elastase activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27260
-
predicted from cDNA clone Rattus norvegicus
29000
-
SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Somatostatin-14 + H2O Rattus norvegicus hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
rat, male Wistar
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no carbohydrate moiety detected in the structure Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 118.83, activity of the enzyme is determined by its ability to degrade 125I-S-14, ng degraded/mg protein Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information elastin-Congo red is no substrate Rattus norvegicus ?
-
?
SMS 201-995 + H2O
-
Rattus norvegicus ?
-
?
Somatostatin-14 + H2O
-
Rattus norvegicus ?
-
?
Somatostatin-14 + H2O hydrolytic enzyme required for intestinal digestion of food stuff, natural substrates are proteins, protein fragments and peptides, greater affinity for natural substrates than for synthetics, specifically involved in the regulation of biological functions of somatostatin-14 in the gastrointestinal tract Rattus norvegicus ?
-
?
Suc-Ala-Ala-Pro-Met-p-nitroanilide + H2O
-
Rattus norvegicus p-nitroaniline + Suc-Ala-Ala-Pro-Met
-
?
Suc-Ala-Ala-Val-Ala-p-nitroanilide + H2O
-
Rattus norvegicus p-nitroaniline + Suc-Ala-Ala-Val-Ala
-
?
Suc-Tyr-Leu-Val-p-nitroanilide + H2O
-
Rattus norvegicus p-nitroaniline + Suc-Tyr-Leu-Val
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Rattus norvegicus

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus isoelectric focusing 5.9 5.6