BRENDA - Enzyme Database
show all sequences of 3.4.21.70

Human pancreatic enzymes: purification and characterization of a nonelastolytic enzyme, protease E. resembling elastase

Mallory, P.A.; Travis, J.; Biochemistry 14, 722-730 (1975)

Data extracted from this reference:

General Stability
General Stability
Organism
Rapid autolysis at protein concentrations above 0.4 mg/ml, stable below
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Acetyl-Ala-Ala-Ala-AlaCH2Cl
-
Homo sapiens
Acetyl-Ala-Ala-AlaCH2Cl
-
Homo sapiens
Acetyl-Ala-Ala-Phe-AlaCH2Cl
-
Homo sapiens
Acetyl-Ala-Ala-Pro-AlaCH2Cl
-
Homo sapiens
Carboxybenzoyl-Gly-Leu-AlaCH2Cl
-
Homo sapiens
Human serum alpha1-antitrypsin
no inhibition by other naturally occuring inhibitors tested
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.166
-
tert-butyloxycarbonyl-L-alanine 4-nitrophenyl ester
-
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
human, gel filtration
Homo sapiens
30500
-
1 * 30500, human, SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
pancreas
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Storage Stability
Storage Stability
Organism
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Carboxybenzoyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Carboxybenzoyl-L-alanine + 4-nitrophenol
-
-
-
-
casein + H2O
-
29635
Homo sapiens
hydrolyzed casein
-
29635
Homo sapiens
-
Hemoglobin + H2O
-
29635
Homo sapiens
Hydrolyzed hemoglobin
-
29635
Homo sapiens
-
additional information
no activity with: benzoyl-L-arginine ethyl ester, tosyl-L-arginine methyl ester
29635
Homo sapiens
?
-
-
-
-
additional information
no elastolytic activity
29635
Homo sapiens
?
-
-
-
-
Tert-Butyloxycarbonyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Tert-Butyloxycarbonyl-L-alanine + 4-nitrophenol
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 30500, human, SDS-PAGE
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
9.5
casein
Homo sapiens
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
8
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, stable for 3 months, unstable below pH 4.0
Homo sapiens
General Stability (protein specific)
General Stability
Organism
Rapid autolysis at protein concentrations above 0.4 mg/ml, stable below
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Acetyl-Ala-Ala-Ala-AlaCH2Cl
-
Homo sapiens
Acetyl-Ala-Ala-AlaCH2Cl
-
Homo sapiens
Acetyl-Ala-Ala-Phe-AlaCH2Cl
-
Homo sapiens
Acetyl-Ala-Ala-Pro-AlaCH2Cl
-
Homo sapiens
Carboxybenzoyl-Gly-Leu-AlaCH2Cl
-
Homo sapiens
Human serum alpha1-antitrypsin
no inhibition by other naturally occuring inhibitors tested
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.166
-
tert-butyloxycarbonyl-L-alanine 4-nitrophenyl ester
-
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
human, gel filtration
Homo sapiens
30500
-
1 * 30500, human, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
pancreas
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Storage Stability (protein specific)
Storage Stability
Organism
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, pH 4.0-8.0, stable for 3 months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Carboxybenzoyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Carboxybenzoyl-L-alanine + 4-nitrophenol
-
-
-
-
casein + H2O
-
29635
Homo sapiens
hydrolyzed casein
-
29635
Homo sapiens
-
Hemoglobin + H2O
-
29635
Homo sapiens
Hydrolyzed hemoglobin
-
29635
Homo sapiens
-
additional information
no activity with: benzoyl-L-arginine ethyl ester, tosyl-L-arginine methyl ester
29635
Homo sapiens
?
-
-
-
-
additional information
no elastolytic activity
29635
Homo sapiens
?
-
-
-
-
Tert-Butyloxycarbonyl-L-alanine 4-nitrophenyl ester + H2O
-
29635
Homo sapiens
Tert-Butyloxycarbonyl-L-alanine + 4-nitrophenol
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 30500, human, SDS-PAGE
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
9.5
casein
Homo sapiens
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
8
24°C, dilute solution of protein concentration of less than 0.4 mg/ml, stable for 3 months, unstable below pH 4.0
Homo sapiens
Other publictions for EC 3.4.21.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665084
Bieth
-
Pancreatic endopeptidase E ...
Bos taurus, Homo sapiens, Sus scrofa
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1510-1511
2004
-
-
3
2
-
-
6
-
-
-
3
-
-
3
-
-
-
-
-
1
-
-
6
-
-
-
-
-
3
-
-
-
-
3
-
-
-
3
-
2
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
29644
Pignol
Crystallographic study of a cl ...
Sus scrofa
J. Mol. Biol.
252
20-24
1995
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29643
Chapus
Binding of terbium and of an e ...
Bos taurus
J. Biol. Chem.
265
3726-3730
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29639
Moulard
Identification of a procarboxy ...
Homo sapiens
FEBS Lett.
250
166-170
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29640
Cambillau
Subunit III of ruminant procar ...
Bos taurus
FEBS Lett.
232
91-95
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29636
Shen
Primary structure of human pan ...
Homo sapiens
Biochemistry
26
3447-3452
1987
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29641
Sziegoleit
A novel proteinase from human ...
Homo sapiens
Biochem. J.
219
735-742
1984
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
-
-
1
-
2
6
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
-
1
-
2
6
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
29642
Sziegoleit
Purification and characterizat ...
Homo sapiens
Biochem. J.
207
573-582
1982
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29637
Kobayashi
The specificity of porcine pan ...
Sus scrofa
J. Biol. Chem.
256
2460-2465
1981
-
-
-
-
-
2
-
3
-
-
-
1
-
2
-
-
-
-
-
2
-
1
7
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
3
-
-
-
1
-
-
-
-
-
2
-
1
7
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
29638
Kobayashi
Identification of zymogen E in ...
Bos taurus
J. Biol. Chem.
256
2466-2470
1981
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
29635
Mallory
Human pancreatic enzymes: puri ...
Homo sapiens
Biochemistry
14
722-730
1975
-
-
-
-
-
1
6
1
-
-
2
-
-
1
-
-
1
-
-
1
1
1
6
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
6
-
1
-
-
2
-
-
-
-
1
-
1
1
1
6
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-