Activating Compound | Comment | Organism | Structure |
---|---|---|---|
vitamin K | dependent on | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E167A | site-directed mutagenesis, the surface loop residue mutation eliminates the cytoprotective signaling properties of APC without affecting its anticoagulant activity, inability of E167A to exhibit significant protective activity in response to TNF-alpha-induced inflammatory events in endothelial cells | Homo sapiens |
E170A | site-directed mutagenesis, the surface loop residue mutation eliminates the cytoprotective signaling properties of APC without affecting its anticoagulant activity, inability of E170A to exhibit significant protective activity in response to TNF-alpha-induced inflammatory events in endothelial cells | Homo sapiens |
S195A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Protein C inhibitor | inhibits wild-type enzyme and mutants E167A and E170A | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.234 | - |
SpPCa | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.249 | - |
SpPCa | pH 7.4, 37°C, recombinant mutant E167A | Homo sapiens | |
0.258 | - |
SpPCa | pH 7.4, 37°C, recombinant mutant E170A | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
active factor Va + H2O | Homo sapiens | proteolytic inactivation | inactive factor V + domain B | - |
? | |
active factor VIIIa + H2O | Homo sapiens | proteolytic inactivation | inactive factor VIII + domain B | - |
? | |
protease activated receptor 1 + H2O | Homo sapiens | APC exhibits cytoprotective and antiinflammatory activity through the endothelial protein C receptor-dependent cleavage of protease activated receptor 1, PAR-1, on endothelial cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
active factor Va + H2O | proteolytic inactivation | Homo sapiens | inactive factor V + domain B | - |
? | |
active factor Va + H2O | proteolytic inactivation, the basic residues of two surface loops including those on 39 and the Ca2+-binding 70-80 loops constitute interactive sites for both factors Va and VIIIa, thereby mediating the interaction of APC specifically with these procoagulant cofactors | Homo sapiens | inactive factor V + domain B | - |
? | |
active factor VIIIa + H2O | proteolytic inactivation | Homo sapiens | inactive factor VIII + domain B | - |
? | |
active factor VIIIa + H2O | proteolytic inactivation, the basic residues of two surface loops including those on 39 and the Ca2+-binding 70-80 loops constitute interactive sites for both factors Va and VIIIa, thereby mediating the interaction of APC specifically with these procoagulant cofactors | Homo sapiens | inactive factor VIII + domain B | - |
? | |
additional information | the enzyme is a vitamin K-dependent serine protease | Homo sapiens | ? | - |
? | |
protease activated receptor 1 + H2O | APC exhibits cytoprotective and antiinflammatory activity through the endothelial protein C receptor-dependent cleavage of protease activated receptor 1, PAR-1, on endothelial cells | Homo sapiens | ? | - |
? | |
protease activated receptor 1 + H2O | activation, the basic residues of surface loops are indispensable for interaction with APC, also two acidic residues on helix-162, Glu167 and Glu170, on the protease domain of APC which are required for the protease interaction with PAR-1 | Homo sapiens | ? | - |
? | |
S2238 + H2O | a commercial chromogenic substrate | Homo sapiens | ? | - |
? | |
SpPCa + H2O | a commercial tripeptidyl chromogenic substrate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Activated protein C | - |
Homo sapiens |
APC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
anticoagulation assay at | Homo sapiens |
37 | - |
amidohydrolytic assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.1 | - |
SpPCa | pH 7.4, 37°C, recombinant wild-type enzyme | Homo sapiens | |
34 | - |
SpPCa | pH 7.4, 37°C, recombinant mutant E167A | Homo sapiens | |
35 | - |
SpPCa | pH 7.4, 37°C, recombinant mutant E170A | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |