Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.69 extracted from

  • Feistritzer, C.; Schuepbach, R.A.; Mosnier, L.O.; Bush, L.A.; Di Cera, E.; Griffin, J.H.; Riewald, M.
    Protective signaling by activated protein C is mechanistically linked to protein C activation on endothelial cells (2006), J. Biol. Chem., 281, 20077-20084.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
S360A site-directed mutagenesis, catalytic site residue mutation, mutant thrombin W215A/E217A induces protective signaling only in the presence of wild type protein C but not mutant protein C S360A Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information wild-type inactive protein C and inactive protein C mutant S360A inhibit the binding of activated protein C to the endothelial protein C receptor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the activated protein C occurs at the cell surfaceof endothelial cells Homo sapiens 9986
-
extracellular
-
Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens activated protein C has endothelial barrier protective effects that require binding to endothelial protein C receptor, EPCR, and cleavage of protease activated receptor-1, PAR1, and that may play a role in the anti-inflammatory action of APC, thrombin does not exert directly barrier protective effects, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification protein C zymogen activation by thrombin on the endothelial cell surface, the double mutant thrombin W215A/E217A is also active in protein C activation and produces APC with high barrier protective activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation APC from plasma Homo sapiens
-
plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information activated protein C has endothelial barrier protective effects that require binding to endothelial protein C receptor, EPCR, and cleavage of protease activated receptor-1, PAR1, and that may play a role in the anti-inflammatory action of APC, thrombin does not exert directly barrier protective effects, overview Homo sapiens ?
-
?
S-2366 + H2O synthetic substrate for amidolytic activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Activated protein C
-
Homo sapiens
APC
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Homo sapiens